Difference: CemSamplePrepWeb (1 vs. 9)

Revision 903 May 2013 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

solarus.jpg cp3.jpg hpf.jpg
Changed:
<
<
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

Bal-Tec HPM 010
High Pressure Freezing Machine

Wohlwend HPF Compact 01
>
>
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

Bal-Tec HPM 010
High Pressure Freezing Machine

Wohlwend HPF Compact 01
 
edwards.jpg microtome.jpg beckman.jpg
Evaporator

BOC Edwards
Auto 306

Ultramicrotome

Leica EM
UC6

Centrifuges

Beckman Optima TLX Ultracentrifuge

Beckman Avanti J-25 Centrifuge





  • Set ALLOWTOPICVIEW =

Revision 803 May 2013 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

solarus.jpg cp3.jpg hpf.jpg
Changed:
<
<
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

_Bal-Tec HPM 010_
High Pressure Freezing Machine

Wohlwend HPF Compact 01
>
>
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

Bal-Tec HPM 010
High Pressure Freezing Machine

Wohlwend HPF Compact 01
 
edwards.jpg microtome.jpg beckman.jpg
Evaporator

BOC Edwards
Auto 306

Ultramicrotome

Leica EM
UC6

Centrifuges

Beckman Optima TLX Ultracentrifuge

Beckman Avanti J-25 Centrifuge





  • Set ALLOWTOPICVIEW =

Revision 703 May 2013 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

solarus.jpg cp3.jpg hpf.jpg
Changed:
<
<
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

Bal-Tec HPM 010
High Pressure Freezing Machine

Wohlwend HPF Compact 01
>
>
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

_Bal-Tec HPM 010_
High Pressure Freezing Machine

Wohlwend HPF Compact 01
 
edwards.jpg microtome.jpg beckman.jpg
Evaporator

BOC Edwards
Auto 306

Ultramicrotome

Leica EM
UC6

Centrifuges

Beckman Optima TLX Ultracentrifuge

Beckman Avanti J-25 Centrifuge





  • Set ALLOWTOPICVIEW =

Revision 619 Sep 2012 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

Changed:
<
<
solarus.jpg cp3.jpg hpf.jpg
>
>
solarus.jpg cp3.jpg hpf.jpg
 
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

Bal-Tec HPM 010
High Pressure Freezing Machine

Wohlwend HPF Compact 01

edwards.jpg microtome.jpg beckman.jpg
Evaporator

BOC Edwards
Auto 306

Ultramicrotome

Leica EM
UC6

Centrifuges

Beckman Optima TLX Ultracentrifuge

Beckman Avanti J-25 Centrifuge





  • Set ALLOWTOPICVIEW =

Revision 530 Aug 2012 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

solarus.jpg cp3.jpg hpf.jpg
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

Bal-Tec HPM 010
High Pressure Freezing Machine

Wohlwend HPF Compact 01

edwards.jpg microtome.jpg beckman.jpg
Evaporator

BOC Edwards
Auto 306

Ultramicrotome

Leica EM
UC6

Centrifuges

Beckman Optima TLX Ultracentrifuge

Beckman Avanti J-25 Centrifuge





Changed:
<
<
Twiki Source.
>
>
 
  • Set ALLOWTOPICVIEW =

Revision 414 Jun 2012 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

solarus.jpg cp3.jpg hpf.jpg
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

Bal-Tec HPM 010
High Pressure Freezing Machine

Wohlwend HPF Compact 01

edwards.jpg microtome.jpg beckman.jpg
Evaporator

BOC Edwards
Auto 306

Ultramicrotome

Leica EM
UC6

Centrifuges

Beckman Optima TLX Ultracentrifuge

Beckman Avanti J-25 Centrifuge
Added:
>
>




Twiki Source.
 
  • Set ALLOWTOPICVIEW =

Deleted:
<
<

Revision 308 Jun 2012 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

solarus.jpg cp3.jpg hpf.jpg
Changed:
<
<
Plasma cleaner Plunge Freezer High Pressure Freezer
>
>
Plasma cleaner

Gatan Solarus
Model 950

Plunge Freezer

Gatan Cp3
Cryoplunge 3

Custom vitrobox
High Pressure Freezer

Bal-Tec HPM 010
High Pressure Freezing Machine

Wohlwend HPF Compact 01
 
edwards.jpg microtome.jpg beckman.jpg
Changed:
<
<
Evaporator Ultramicrotome Ultracentrifuge
>
>
Evaporator

BOC Edwards
Auto 306

Ultramicrotome

Leica EM
UC6

Centrifuges

Beckman Optima TLX Ultracentrifuge

Beckman Avanti J-25 Centrifuge
 

  • Set ALLOWTOPICVIEW =

Revision 218 May 2012 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

Changed:
<
<
solarus.jpg cp3.jpg
Plasma cleaner Plunge Freezer
   
hpf.jpg edwards.jpg
High Pressure Freezer Evaporator
>
>
solarus.jpg cp3.jpg hpf.jpg
Plasma cleaner Plunge Freezer High Pressure Freezer

edwards.jpg microtome.jpg beckman.jpg
Evaporator Ultramicrotome Ultracentrifuge
Deleted:
<
<
   
microtome.jpg beckman.jpg
Ultramicrotome Ultracentrifuge
 
Added:
>
>
 
  • Set ALLOWTOPICVIEW =

Added:
>
>

Revision 118 May 2012 - Main.EdEng

 
META TOPICPARENT name="CemTwikiToWebMainPage"

Sample preparation

In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology community. For protein solutions we have a fully equipped wet laboratory, where we keep stocks of heavy metal salts that can be used as negative stains. In addition, we have an evaporator to prepare carbon support films on mica sheets, or to evaporate carbon on a plastic substrate. Frozen hydrated samples are easily prepared using either a manual, home-made device, or a new Gatan CP3 plunger, which is easy to use by newcomers.

Tissue samples can be prepared for structural studies using high pressure freezing and freeze substitution, followed by resin embedding and sectioning on a Leica ultramicrotome. High pressure frozen samples can also be sectioned directly on the ultramicrotome with the cryogenic attachment.

Of course, ultracentrifugation and some (limited) protein purification capabilities are also available.

solarus.jpg cp3.jpg
Plasma cleaner Plunge Freezer
   
hpf.jpg edwards.jpg
High Pressure Freezer Evaporator
   
microtome.jpg beckman.jpg
Ultramicrotome Ultracentrifuge

  • Set ALLOWTOPICVIEW =

 
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