Optical Bench and Scanner Instructions

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

Protocol

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. 15 minutes in developer
5. 1 minute rinse (first pair of water tanks)
6. 10 minute fix
7. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo
9. Remove from water
10. Label racks with your name and the date
11. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

Changing Film

• See each individual microscope page for specific instructions
• Special Film Desiccator Instructions:
  • Make sure you change the Phosphorus Pentoxide each time you change cartridge
  • Place container of Phosphorus Pentoxide in fume hood NYSBC staff will dispose of properly
• When changing film cartridge more than once in a day
  • Be aware wet film prevents cryo cycle from running, to prevent staying late while film pumps down:
    • Make sure you change first film cartridge before noon
    • Make arrangement with NYSBC staff to load dry film in the morning after your session and load empty film cartridge

Mixing Dark Room Chemicals

• Good for 600 films or 2 months
• Change chemicals
  • when the optical density is bad
  • testing film/microscope
  • critical samples

Developer

• Mix full strength D-19 developer
• Prepare at least one day in advance
  • do not use warm

Fixer

• use full strength
• pour contents from bottle
  • put remainder of contenets in a compressed air bottle to minimize air contact

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. 15 minutes in developer
5. 1 minute rinse (first pair of water tanks)
6. 10 minute fix
7. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo
9. Remove from water
10. Label racks with your name and the date
11. Place on metal shelves to dry

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• See each individual microscope page for specific instructions
• Special Film Desiccator Instructions:
  • Make sure you change the Phosphorus Pentoxide each time you change cartridge
  • Place container of Phosphorus Pentoxide in fume hood NYSBC staff will dispose of properly
• When changing film cartridge more than once in a day
  • Be aware wet film prevents cryo cycle from running, to prevent staying late while film pumps down:
    • Make sure you change first film cartridge before noon
    • Make arrangement with NYSBC staff to load dry film in the morning after your session and load empty film cartridge

• Good for 600 films or 2 months
• Change chemicals
  • when the optical density is bad
  • testing film/microscope
  • critical samples

• Mix full strength D-19 developer
• Prepare at least one day in advance
  • do not use warm

• use full strength
• pour contents from bottle
  • put remainder of contenets in a compressed air bottle to minimize air contact

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

Protocol

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. 15 minutes in developer
5. 1 minute rinse (first pair of water tanks)
6. 10 minute fix
7. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo
9. Remove from water
10. Label racks with your name and the date
11. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

Changing Film

• See each individual microscope page for specific instructions
• Special Film Desiccator Instructions:
  • Make sure you change the Phosphorus Pentoxide each time you change cartridge
  • Place container of Phosphorus Pentoxide in fume hood NYSBC staff will dispose of properly
• When changing film cartridge more than once in a day
  • Be aware wet film prevents cryo cycle from running, to prevent staying late while film pumps down:
    • Make sure you change first film cartridge before noon
    • Make arrangement with NYSBC staff to load dry film in the morning after your session and load empty film cartridge

Mixing Dark Room Chemicals

• Good for 600 films or 2 months
• Change chemicals
  • when the optical density is bad
  • testing film/microscope
  • critical samples

Developer

• Mix full strength D-19 developer
• Prepare at least one day in advance
  • do not use warm

Fixer

• use full strength
• pour contents from bottle
  • put remainder of contenets in a compressed air bottle to minimize air contact

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. 15 minutes in developer
5. 1 minute rinse (first pair of water tanks)
6. 10 minute fix
7. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo
9. Remove from water
10. Label racks with your name and the date
11. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• See each individual microscope page for specific instructions
• Special Film Desiccator Instructions:
  • Make sure you change the Phosphorus Pentoxide each time you change cartridge
  • Place container of Phosphorus Pentoxide in fume hood NYSBC staff will dispose of properly
• When changing film cartridge more than once in a day
  • Be aware wet film prevents cryo cycle from running, to prevent staying late while film pumps down:
    • Make sure you change first film cartridge before noon
    • Make arrangement with NYSBC staff to load dry film in the morning after your session and load empty film cartridge

• Good for 600 films or 2 months
• Change chemicals
  • when the optical density is bad
  • testing film/microscope
  • critical samples

• Mix full strength D-19 developer
• Prepare at least one day in advance
  • do not use warm

• use full strength
• pour contents from bottle
  • put remainder of contenets in a compressed air bottle to minimize air contact

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. 15 minutes in developer
5. 1 minute rinse (first pair of water tanks)
6. 10 minute fix
7. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo
9. Remove from water
10. Label racks with your name and the date
11. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

Changing Film

• See each individual microscope page for specific instructions
• Special Film Desiccator Instructions:
  • Make sure you change the Phosphorus Pentoxide each time you change cartridge
  • Place container of Phosphorus Pentoxide in fume hood NYSBC staff will dispose of properly
• When changing film cartridge more than once in a day
  • Be aware wet film prevents cryo cycle from running, to prevent staying late while film pumps down:
    • Make sure you change first film cartridge before noon
    • Make arrangement with NYSBC staff to load dry film in the morning after your session and load empty film cartridge

Mixing Dark Room Chemicals

• Good for 600 films or 2 months
• Change chemicals
  • when the optical density is bad
  • testing film/microscope
  • critical samples

Developer

• Mix full strength D-19 developer
• Prepare at least one day in advance
  • do not use warm

Fixer

• use full strength
• pour contents from bottle
  • put remainder of contenets in a compressed air bottle to minimize air contact

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. 15 minutes in developer
5. 1 minute rinse (first pair of water tanks)
6. 10 minute fix
7. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo
9. Remove from water
10. Label racks with your name and the date
11. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• See each individual microscope page for specific instructions
• Special Film Desiccator Instructions:
  • Make sure you change the Phosphorus Pentoxide each time you change cartridge
  • Place container of Phosphorus Pentoxide in fume hood NYSBC staff will dispose of properly
• When changing film cartridge more than once in a day
  • Be aware wet film prevents cryo cycle from running, to prevent staying late while film pumps down:
    • Make sure you change first film cartridge before noon
    • Make arrangement with NYSBC staff to load dry film in the morning after your session and load empty film cartridge

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

Procedure

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. 15 minutes in developer
5. 1 minute rinse (first pair of water tanks)
6. 10 minute fix
7. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo
9. Remove from water
10. Label racks with your name and the date
11. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

Procedure

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. 15 minutes in developer
5. 1 minute rinse (first pair of water tanks)
6. 10 minute fix
7. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo
9. Remove from water
10. Label racks with your name and the date
11. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

You Will Need

• Microscope Film Cassette
• SO - 163 Undeveloped Film
• Film Racks

Procedure

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.

1. 1 minute rinse (first pair of water tanks)
2. 10 minute fix
3. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
4. Add a few drops of photo-flo
5. Remove from water
6. Label racks with your name and the date
7. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

You Will Need

• Microscope Film Cassette
• SO - 163 Undeveloped Film
• Film Racks

Procedure

1. Turn on red safe light by flipping switch down.
2. Fill the water tanks
3. Insert film into racks.
4. Place film in developer tank for 15 minutes
5. 1 minute rinse (first pair of water tanks)

1. 30 minute running water rinse (second pair of water tanks)
   • (Note:At this point it is safe to turn on the normal light in the room)
2. Add a few drops of photo-flo
3. Remove from water
4. Label racks with your name and the date
5. Place on metal shelves to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. Load film notch in the upper right hand corner into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

You Will Need

• Microscope Film Cassette

• Film Racks

Procedure

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film

1. Carefully place the plates back into the cassette and close the lid

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

You Will Need

• Microscope Film Cassette
• Undeveloped Film
• Film Racks

Procedure

1. Flip light switch down to turn on red safe light.
2. Remove the film from the plates and insert them into film racks.
3. Fill the water tanks to the top
4. Place film racks in developer tanks for 15 minutes
5. Rinse film racks in first pair of water tanks for 1 minute
6. Move film racks to fixer tanks for 10 minutes
7. Rinse film racks in second pair of water tanks with running water for 30 minutes (Note:At this point it is safe to turn on the normal light in the room)
8. Add a few drops of photo-flo and rinse film racks for 1 minute
9. Remove film racks from water
10. Label film racks with your name and the date
11. Place film racks on one of the metal shelves in the darkroom and allow them to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. With the notch in the upper right hand corner of the undeveloped film, load them into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

You Will Need

• Microscope Film Cassette
• Undeveloped Film
• Film Racks

Procedure

1. Flip light switch down to turn on red safe light.
2. Remove the film from the plates and insert them into film racks.
3. Fill the water tanks to the top
4. Place film racks in developer tanks for 15 minutes
5. Rinse film racks in first pair of water tanks for 1 minute
6. Move film racks to fixer tanks for 10 minutes

1. Add a few drops of photo-flo and rinse film racks for 1 minute
2. Remove film racks from water
3. Label film racks with your name and the date
4. Place film racks on one of the metal shelves in the darkroom and allow them to dry

While your film is developing

1. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
2. With the notch in the upper right hand corner of the undeveloped film, load them into empty plates.
3. Carefully place the plates back into the cassette and close the lid

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

WEAR GLOVES!!!

You Will Need

• Microscope Film Cassette
• Undeveloped Film
• Film Racks

Procedure

1. Flip light switch down to turn on red safe light.

While your film is developing

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

You Will Need

• Microscope Film Cassette
• Undeveloped Film
• Film Racks

Procedure

1. Flip light switch down to turn on red safe light.
2. Remove the film from the plates and insert them into film racks.
3. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
4. Load the plates with undeveloped film
5. Carefully place the plates back into the cassette and close the lid
6. Fill the water tanks to the top
7. Place film racks in developer tanks for 15 minutes
8. Rinse film racks in first pair of water tanks for 1 minute
9. Move film racks to fixer tanks for 10 minutes
10. Rinse film racks in second pair of water tanks for 30 minutes (Note:During this period, running water should be applied to the water tanks. At this point it is safe to turn on the normal light in the room)
11. Add a few drops of photo-flo and rinse film racks for 1 minute
12. Remove film racks from water
13. Label film racks with your name and the date
14. Place film racks on one of the metal shelves in the darkroom and allow them to dry

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

You Will Need

• Microscope Film Cassette
• Undeveloped Film
• Film Racks

Procedure

1. Flip light switch down to turn on red safe light.

1. Remove the film from the plates and insert them into film racks.
2. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
3. Load the plates with undeveloped film
4. Carefully place the plates back into the cassette and close the lid
5. Fill the water tanks to the top
6. Place film racks in developer tanks for 15 minutes
7. Rinse film racks in first pair of water tanks for 1 minute
8. Move film racks to fixer tanks for 10 minutes
9. Rinse film racks in second pair of water tanks for 30 minutes (Note:During this period, running water should be applied to the water tanks. At this point it is safe to turn on the normal light in the room)
10. Add a few drops of photo-flo and rinse film racks for 1 minute
11. Remove film racks from water
12. Label film racks with your name and the date
13. Place film racks on one of the metal shelves in the darkroom and allow them to dry

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

You Will Need

Procedure

1. Flip light switch down to turn on red safe light.
2. Unload film plates from cassette
3. Remove the film from the plates and insert them into film racks.
4. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
5. Load the plates with undeveloped film
6. Carefully place the plates back into the cassette and close the lid
7. Fill the water tanks to the top
8. Place film racks in developer tanks for 15 minutes
9. Rinse film racks in first pair of water tanks for 1 minute
10. Move film racks to fixer tanks for 10 minutes
11. Rinse film racks in second pair of water tanks for 30 minutes (Note:During this period, running water should be applied to the water tanks. At this point it is safe to turn on the normal light in the room)
12. Add a few drops of photo-flo and rinse film racks for 1 minute
13. Remove film racks from water
14. Label film racks with your name and the date
15. Place film racks on one of the metal shelves in the darkroom and allow them to dry

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

You Will Need

Procedure

1. Flip light switch down to turn on red safe light.
2. Unload film plates from cassette
3. Remove the film from the plates and insert them into film racks.
4. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
5. Load the plates with undeveloped film
6. Carefully place the plates back into the cassette and close the lid
7. Fill the water tanks to the top
8. Place film racks in developer tanks for 15 minutes
9. Rinse film racks in first pair of water tanks for 1 minute
10. Move film racks to fixer tanks for 10 minutes

1. Add a few drops of photo-flo and rinse film racks for 1 minute
2. Remove film racks from water
3. Label film racks with your name and the date
4. Place film racks on one of the metal shelves in the darkroom and allow them to dry

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

You Will Need

Procedure

1. Flip light switch down to turn on red safe light.
2. Unload film plates from cassette
3. Remove the film from the plates and insert them into film racks.
4. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
5. Load the plates with undeveloped film
6. Carefully place the plates back into the cassette and close the lid
7. Fill the water tanks to the top
8. Place film racks in developer tanks for 15 minutes
9. Rinse film racks in first pair of water tanks for 1 minute
10. Move film racks to fixer tanks for 10 minutes
11. Rinse film racks in second pair of water tanks for 30 minutes

1. Add a few drops of photo-flo and rinse film racks for 1 minute
2. Remove film racks from water
3. Label film racks with your name and the date
4. Place film racks on one of the metal shelves in the darkroom and allow them to dry

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

You Will Need

Procedure

1. Flip light switch down to turn on red safe light.
2. Unload film plates from cassette
3. Remove the film from the plates and insert them into film racks.
4. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
5. Load the plates with undeveloped film
6. Carefully place the plates back into the cassette and close the lid
7. Fill the water tanks to the top
8. Place film racks in developer tanks for 15 minutes
9. Rinse film racks in first pair of water tanks for 1 minute
10. Move film racks to fixer tanks for 10 minutes
11. Rinse film racks in second pair of water tanks for 30 minutes *Note:During this period, running water should be applied to the water tanks. At this point it is safe to turn on the normal light in the room
12. Add a few drops of photo-flo and rinse film racks for 1 minute
13. Remove film racks from water
14. Label film racks with your name and the date
15. Place film racks on one of the metal shelves in the darkroom and allow them to dry

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

You Will Need

Procedure

1. Flip light switch down to turn on red safe light.
2. Unload film plates from cassette
3. Remove the film from the plates and insert them into film racks.
4. Open the box and light-tight foil packing labeled "SO-163" and remove the undeveloped film
5. Load the plates with undeveloped film
6. Carefully place the plates back into the cassette and close the lid
7. Fill the water tanks to the top
8. Place film racks in developer tanks for 15 minutes
9. Rinse film racks in first pair of water tanks for 1 minute
10. Move film racks to fixer tanks for 10 minutes
11. Rinse film racks in second pair of water tanks for 30 minutes

1. Add a few drops of photo-flo and rinse film racks for 1 minute
2. Remove film racks from water
3. Label film racks with your name and the date
4. Place film racks on one of the metal shelves in the darkroom and allow them to dry

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007

Darkroom Protocols

Developing Film

1. Make sure that only safe red lights are on
2. Place micrograph rack in developer tanks and leave for 12 minutes. This allows the formation of crystals to the point of saturation. After this point, there is no crystal formation, but leaving the film in the developer tank does not really damage the film, so long as it is not kept in for long periods of time.
3. Rinse the film in the two water tanks next to the developer tanks for around 1 minute, just to remove as much developer as possible.
4. Move the film to the fixer tanks and leave there for about 10 minutes. After approximately 6-7 minutes, the film should be fixed as most of the silver bromide in the film should be removed, but leaving it in the tanks for a full 10 minutes insures that it has become fixed and does not affect film quality. Leaving the film in the fixer tanks for too long can cause the film to yellow because of the way the fixer reacts with the plastic on the film.
5. Rinse the film in water for 30 minutes or so, using a few drops of photo-flo at the end of this period to remove water from the film.

• Set ALLOWTOPICVIEW =

-- MichaelStaropoli - 30 Nov 2007