Difference: DetergentAndLipidScreening (1 vs. 7)

Revision 731 Aug 2011 - Main.NathanWill

 
META TOPICPARENT name="TemimpsGroup"

Detergent and lipid screens

Contents

MCMJR1

MCMJR1 FC-12 Lipid Stability Screen

MHJF1

MHJF1 FC-12 Lipid Stability Screen

UMA

UMA FC-12 and DDM purification trials

  • UMA was purified with either FC-12 or DDM to determine stability and yield of UMA with each detergent:
  • UMA_3-1-11_Exp_Trial.ppt: UMA_3-1-11_Exp_Trial.ppt

APB

APB FC-12 and DDM purification trials

  • APB purified in either FC-12 of DDM including ULP1 treatment to determine stability and yield of UMA with each detergent:
  • APB_3-1-11_Exp_Trial.ppt: APB_3-1-11_Exp_Trial.ppt

  • APB Detergent screen (APB solubilized in DDM and sent for NYCOMPS detergent screen)
  • APB_Detergent_Screen.jpg: NYCOMPS Detergent screen of APB

Nycomps Detergent Screen

  • APB Detergent screen (APB solubilized in DDM and sent for NYCOMPS detergent screen)
  • APB_Detergent_Screen.jpg: NYCOMPS Detergent screen of APB

  • MHJF1 Detergent screen (MHJF1 solubilized in DDM and sent for NYCOMPS detergent screen)
  • MHJF1_061711.jpg: NYCOMPS Detergent screen of MHJF1

  • MHJF1 Detergent screen (MHJF1 solubilized in DDM and sent for NYCOMPS detergent screen)
  • MCMJR1_061711.jpg: NYCOMPS Detergent screen of MCMJR1

  • Set ALLOWTOPICVIEW =

-- NathanWill - 24 Aug 2011

META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 NYCOMPS Detergent screen (purified in DDM)" date="1314722569" name="MHJF1_061711.jpg" path="MHJF1_061711.jpg" size="3005469" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="UAM purification trial in FC-12 or DDM" date="1314214278" name="UMA_3-1-11_Exp_Trial.ppt" path="UMA_3-1-11_Exp_Trial.ppt" size="1982464" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150" date="1314196265" name="7-26-11_Lipid_Screen.ppt" path="7-26-11_Lipid_Screen.ppt" size="364032" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="APB purification trial comparing FC-12 and DDM" date="1314214562" name="APB_3-1-11_Exp_Trial.ppt" path="APB_3-1-11_Exp_Trial.ppt" size="1932288" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150" date="1314194372" name="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" path="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" size="1077248" user="Main.NathanWill" version="1"
Changed:
<
<
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314720412" name="APB_detergent_screen_041511.jpg" path="APB_detergent_screen_041511.jpg" size="2719909" user="Main.NathanWill" version="1"
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="APB NYCOMPS Detergent screen (purified in DDM)" date="1314720412" name="APB_detergent_screen_041511.jpg" path="APB_detergent_screen_041511.jpg" size="2719909" user="Main.NathanWill" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 NYCOMPS Detergent screen (Purified in DDM)" date="1314722773" name="MCMJR1_061711.jpg" path="MCMJR1_061711.jpg" size="3141842" user="Main.NathanWill" version="1"

Revision 630 Aug 2011 - Main.NathanWill

 
META TOPICPARENT name="TemimpsGroup"

Detergent and lipid screens

Contents

MCMJR1

MCMJR1 FC-12 Lipid Stability Screen

MHJF1

MHJF1 FC-12 Lipid Stability Screen

UMA

UMA FC-12 and DDM purification trials

  • UMA was purified with either FC-12 or DDM to determine stability and yield of UMA with each detergent:
  • UMA_3-1-11_Exp_Trial.ppt: UMA_3-1-11_Exp_Trial.ppt

APB

APB FC-12 and DDM purification trials

  • APB purified in either FC-12 of DDM including ULP1 treatment to determine stability and yield of UMA with each detergent:
Changed:
<
<		 * APB_3-1-11_Exp_Trial.ppt: APB_3-1-11_Exp_Trial.ppt
>
>
 
Added:
>
>
  • APB Detergent screen (APB solubilized in DDM and sent for NYCOMPS detergent screen)
  • APB_Detergent_Screen.jpg: NYCOMPS Detergent screen of APB
 
Changed:
<
<		d38 3
  • Set ALLOWTOPICVIEW =
>
>

Nycomps Detergent Screen

  • APB Detergent screen (APB solubilized in DDM and sent for NYCOMPS detergent screen)
Added:
>
>
 
Changed:
<
<		-- NathanWill - 24 Aug 2011
>
>
  • MHJF1 Detergent screen (MHJF1 solubilized in DDM and sent for NYCOMPS detergent screen)
Added:
>
>
 
Added:
>
>
  • MHJF1 Detergent screen (MHJF1 solubilized in DDM and sent for NYCOMPS detergent screen)
  • MCMJR1_061711.jpg: NYCOMPS Detergent screen of MCMJR1
 
Added:
>
>
  • Set ALLOWTOPICVIEW =
 
Changed:
<
<
>
>		-- NathanWill - 24 Aug 2011
 
Changed:
<
<		 * APB_detergent_screen_041511.jpg:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 NYCOMPS Detergent screen (purified in DDM)" date="1314722569" name="MHJF1_061711.jpg" path="MHJF1_061711.jpg" size="3005469" user="Main.NathanWill" version="1"
Deleted:
<
<		 APB_detergent_screen_041511.jpg d38 1
 
META FILEATTACHMENT attr="" autoattached="1" comment="UAM purification trial in FC-12 or DDM" date="1314214278" name="UMA_3-1-11_Exp_Trial.ppt" path="UMA_3-1-11_Exp_Trial.ppt" size="1982464" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150" date="1314196265" name="7-26-11_Lipid_Screen.ppt" path="7-26-11_Lipid_Screen.ppt" size="364032" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="APB purification trial comparing FC-12 and DDM" date="1314214562" name="APB_3-1-11_Exp_Trial.ppt" path="APB_3-1-11_Exp_Trial.ppt" size="1932288" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150" date="1314194372" name="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" path="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" size="1077248" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314720412" name="APB_detergent_screen_041511.jpg" path="APB_detergent_screen_041511.jpg" size="2719909" user="Main.NathanWill" version="1"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 NYCOMPS Detergent screen (Purified in DDM)" date="1314722773" name="MCMJR1_061711.jpg" path="MCMJR1_061711.jpg" size="3141842" user="Main.NathanWill" version="1"
 

Revision 524 Aug 2011 - Main.NathanWill

 
META TOPICPARENT name="TemimpsGroup"

Detergent and lipid screens

Contents

MCMJR1

MCMJR1 FC-12 Lipid Stability Screen

Added:
>
>
 
Deleted:
<
<
* Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt: MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150
*

 

MHJF1

MHJF1 FC-12 Lipid Stability Screen

Changed:
<
<
* 7-26-11_Lipid_Screen.ppt: MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150
>
>
 
Added:
>
>

UMA

UMA FC-12 and DDM purification trials

  • UMA was purified with either FC-12 or DDM to determine stability and yield of UMA with each detergent:
  • UMA_3-1-11_Exp_Trial.ppt: UMA_3-1-11_Exp_Trial.ppt

APB

APB FC-12 and DDM purification trials

  • APB purified in either FC-12 of DDM including ULP1 treatment to determine stability and yield of UMA with each detergent: * APB_3-1-11_Exp_Trial.ppt: APB_3-1-11_Exp_Trial.ppt

d38 3

 
  • Set ALLOWTOPICVIEW =

-- NathanWill - 24 Aug 2011

Added:
>
>

* APB_detergent_screen_041511.jpg:

  APB_detergent_screen_041511.jpg
Added:
>
>		d38 1
 
META FILEATTACHMENT attr="" autoattached="1" comment="UAM purification trial in FC-12 or DDM" date="1314214278" name="UMA_3-1-11_Exp_Trial.ppt" path="UMA_3-1-11_Exp_Trial.ppt" size="1982464" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150" date="1314196265" name="7-26-11_Lipid_Screen.ppt" path="7-26-11_Lipid_Screen.ppt" size="364032" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="APB purification trial comparing FC-12 and DDM" date="1314214562" name="APB_3-1-11_Exp_Trial.ppt" path="APB_3-1-11_Exp_Trial.ppt" size="1932288" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150" date="1314194372" name="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" path="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" size="1077248" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314720412" name="APB_detergent_screen_041511.jpg" path="APB_detergent_screen_041511.jpg" size="2719909" user="Main.NathanWill" version="1"

Revision 424 Aug 2011 - Main.NathanWill

 
META TOPICPARENT name="TemimpsGroup"

Detergent and lipid screens

Contents

Deleted:
<
<		d9 1
 

MCMJR1

MCMJR1 FC-12 Lipid Stability Screen

Deleted:
<
<

Purifications of MCMJR1

 
Changed:
<
<
>
>
* Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt: MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150
Added:
>
>
*

MHJF1

MHJF1 FC-12 Lipid Stability Screen

 
Added:
>
>
* 7-26-11_Lipid_Screen.ppt: MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150
 
Deleted:
<
<		d16 1
 
  • Set ALLOWTOPICVIEW =

-- NathanWill - 24 Aug 2011

Deleted:
<
<		-- NicolasCoudray - 09 Aug 2011
 
Changed:
<
<
>
>		 APB_detergent_screen_041511.jpg
META FILEATTACHMENT attr="" autoattached="1" comment="UAM purification trial in FC-12 or DDM" date="1314214278" name="UMA_3-1-11_Exp_Trial.ppt" path="UMA_3-1-11_Exp_Trial.ppt" size="1982464" user="Main.NathanWill" version="1"
Deleted:
<
<
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314025608" name="5-7-11_Purification_Data.ppt" path="5-7-11_Purification_Data.ppt" size="1004544" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150" date="1314196265" name="7-26-11_Lipid_Screen.ppt" path="7-26-11_Lipid_Screen.ppt" size="364032" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="APB purification trial comparing FC-12 and DDM" date="1314214562" name="APB_3-1-11_Exp_Trial.ppt" path="APB_3-1-11_Exp_Trial.ppt" size="1932288" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150" date="1314194372" name="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" path="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" size="1077248" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314720412" name="APB_detergent_screen_041511.jpg" path="APB_detergent_screen_041511.jpg" size="2719909" user="Main.NathanWill" version="1"

Revision 323 Aug 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="TemimpsGroup"

Detergent and lipid screens

Contents

d9 1

Changed:
<
<

Name of the topic

Sublevel topic

>
>

MCMJR1

MCMJR1 FC-12 Lipid Stability Screen

Deleted:
<
<

Purifications of MCMJR1

 

Purifications of MCMJR1

Changed:
<
<		5-7-11 Purification tests of MCMJR1 in DDM and FC12: 5-7-11_Purification_Data.ppt.
>
>
 

d16 1

  • Set ALLOWTOPICVIEW =

-- NathanWill - 24 Aug 2011 -- NicolasCoudray - 09 Aug 2011

Changed:
<
<
META FILEATTACHMENT attachment="5-7-11_Purification_Data.ppt" attr="" comment="" date="1314025608" name="5-7-11_Purification_Data.ppt" path="5-7-11 Purification Data.ppt" size="1004544" stream="5-7-11 Purification Data.ppt" user="Main.NicolasCoudray" version="1"
>
>
Added:
>
>
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314025608" name="5-7-11_Purification_Data.ppt" path="5-7-11_Purification_Data.ppt" size="1004544" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150" date="1314196265" name="7-26-11_Lipid_Screen.ppt" path="7-26-11_Lipid_Screen.ppt" size="364032" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="APB purification trial comparing FC-12 and DDM" date="1314214562" name="APB_3-1-11_Exp_Trial.ppt" path="APB_3-1-11_Exp_Trial.ppt" size="1932288" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150" date="1314194372" name="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" path="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" size="1077248" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314720412" name="APB_detergent_screen_041511.jpg" path="APB_detergent_screen_041511.jpg" size="2719909" user="Main.NathanWill" version="1"

Revision 222 Aug 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="TemimpsGroup"

Detergent and lipid screens

Contents

d9 1

Name of the topic

Sublevel topic

Changed:
<
<

subsub level topic

>
>

Purifications of MCMJR1

 

Purifications of MCMJR1

Added:
>
>		5-7-11 Purification tests of MCMJR1 in DDM and FC12: 5-7-11_Purification_Data.ppt.
 
Added:
>
>
 d16 1
  • Set ALLOWTOPICVIEW =
Added:
>
>		-- NathanWill - 24 Aug 2011 -- NicolasCoudray - 09 Aug 2011
META FILEATTACHMENT attachment="5-7-11_Purification_Data.ppt" attr="" comment="" date="1314025608" name="5-7-11_Purification_Data.ppt" path="5-7-11 Purification Data.ppt" size="1004544" stream="5-7-11 Purification Data.ppt" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314025608" name="5-7-11_Purification_Data.ppt" path="5-7-11_Purification_Data.ppt" size="1004544" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150" date="1314196265" name="7-26-11_Lipid_Screen.ppt" path="7-26-11_Lipid_Screen.ppt" size="364032" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="APB purification trial comparing FC-12 and DDM" date="1314214562" name="APB_3-1-11_Exp_Trial.ppt" path="APB_3-1-11_Exp_Trial.ppt" size="1932288" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150" date="1314194372" name="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" path="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" size="1077248" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314720412" name="APB_detergent_screen_041511.jpg" path="APB_detergent_screen_041511.jpg" size="2719909" user="Main.NathanWill" version="1"

Revision 109 Aug 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="TemimpsGroup"

Detergent and lipid screens

Contents

d9 1

Name of the topic

Sublevel topic

subsub level topic

Purifications of MCMJR1

d16 1

  • Set ALLOWTOPICVIEW =

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314025608" name="5-7-11_Purification_Data.ppt" path="5-7-11_Purification_Data.ppt" size="1004544" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MHJF1 FC-12 Lipid Stability Screen on Superdex200 5/150" date="1314196265" name="7-26-11_Lipid_Screen.ppt" path="7-26-11_Lipid_Screen.ppt" size="364032" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="APB purification trial comparing FC-12 and DDM" date="1314214562" name="APB_3-1-11_Exp_Trial.ppt" path="APB_3-1-11_Exp_Trial.ppt" size="1932288" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="MCMJR1 FC-12 Lipid Stability Screen on superdex200 5/150" date="1314194372" name="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" path="Superdex200_5_150_MCMJR1_Lipid_Screen2.ppt" size="1077248" user="Main.NathanWill" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1314720412" name="APB_detergent_screen_041511.jpg" path="APB_detergent_screen_041511.jpg" size="2719909" user="Main.NathanWill" version="1"
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