Difference: FilmImage (1 vs. 11)

Revision 1130 Oct 2007 - Main.KdDerr

META TOPICPARENT	name="TecnaiInstructions"

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM

Taking a film image

Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
Lift screen
Cover glass viewing chamber
Low Dose mode
- After focusing; while still in Focus mode
- Click exposure found in exposure tab
Other viewing modes
- Under camera menu
- Plate camera (user) menu (pullout)
- Settings Tab

Deleted:

<
<

Electron Dose and film OD

All microscopes will have emulsion settings to provide OD=0.7 for automatic exposure

John Berriman notes regarding film OD
- exposure with 1 e/um2 ( 16.7 pA/cm2) 12 mins Full-Strength D19 gives
  - 80kV: OD 2.2
  - 120kV: OD 2.0
  - 200kV: OD 1.4
  - 300kV: OD 1.0
  - 400kV: OD 0.7

Film speeds (according to Kodak literature with 80 kV electrons)
- factor of 1.0 for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
- factor of 2.75 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water)
  - i.e., need 2.75x longer exposure to give equivalent film OD
  - i.e., auto exp time with Tecnai emulsion setting of 2.8 should give OD of ~0.7
- factor of 5.5 for Kodak 4489 developed in 1:2 dilution of D-19
  - i.e., need 5.5x longer exposure to give equivalent film OD
  - i.e., auto exp time with Tecnai emulsion setting of 5.6 should give OD of ~0.7
- People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution
- Kodak relative speeds (from Kodak literature received 1993) - these values were used to derive above factors
  - Kodak electron image plate in D-19 1:2 dil at 20C for 4 min: 70
  - Kodak 4489 in D-19 1:2 dil at 20C for 4 min: 40
  - Kodak SO-163 in D-19 full-strength + 1g/L benzotriazole (antifog) for 2 min: 15
  - Kodak SO-163 in D-19 1:2 dil at 20C for 4 min: 80
  - Kodak SO-163 in D-19 full-strength at 20C for 4 min: 220
- Grain size goes inversely to film speed

Tecnai F20

Recommended Emulsion settings
- 1.0 cryo samples - this will produce film OD = 0.7 for auto exposure time
- data setting at 1.0 - this will produce reasonably dense numbers with full strength D19 developer
- 1.4 plastic sections - this will produce film OD = 1.0 for auto exposure time

Corresponding film OD and specimen dose at 200kV
- Using SO-163 film developed for 12-15 min in full-strength D19
- Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
- film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
- Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
- Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage
- at 100kV, get an increase in film OD of ~1.6 for the same dose

JEOL2100

Faraday cage used by Rob Hynes to check calibration of the beam current measured from the screen
11 pA/cm2 will produce a film OD=1.0 with 1 sec exposure
emulsion setting of 19 will produce film OD=1.0 with auto exposure time
to calculate specimen dose
- 1A = 1Coulomb/sec
- 1pA = 10^-12C/sec = 10^-12 e^-/1.6x10^-19 sec = 6.25x10⁶ e^-/sec
- 10 pA/cm² = 6.25x10⁷e/sec-cm² * 1 um²/10⁸ A² = 0.625 e^-/sec/um²
- at mag, multiply above result by mag²*1 um²/10⁸A²
  - at 50,000x, 10 pA/cm² corresponds to 15.6 e/A²
    - but Jaap claims a 0.8 mag factor to small screen, suggesting that 10 pA/cm² => 10 e/A²
  - at 60,000x, 10 pA/cm² corresponds to 22.5 e/A²
    - but Jaap claims a 0.8 mag factor to small screen, suggesting that 10 pA/cm² => 14.4 e/A²
CCD counts/electron =

JEOL3200

Gatan engineers claim 17.743 counts/electron
- post-column magnification of 1.3
- pA/cm² *11/1000 = nA total current (pA/cm2 measured on small screen)

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 1022 Oct 2007 - Main.KdDerr

META TOPICPARENT	name="TecnaiInstructions"

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM

Taking a film image

Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
Lift screen
Cover glass viewing chamber
Low Dose mode

Added:

>
>

- After focusing; while still in Focus mode

- Click exposure found in exposure tab
Other viewing modes
- Under camera menu
- Plate camera (user) menu (pullout)
- Settings Tab

Electron Dose and film OD

All microscopes will have emulsion settings to provide OD=0.7 for automatic exposure

John Berriman notes regarding film OD
- exposure with 1 e/um2 ( 16.7 pA/cm2) 12 mins Full-Strength D19 gives
  - 80kV: OD 2.2
  - 120kV: OD 2.0
  - 200kV: OD 1.4
  - 300kV: OD 1.0
  - 400kV: OD 0.7

Film speeds (according to Kodak literature with 80 kV electrons)
- factor of 1.0 for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
- factor of 2.75 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water)
  - i.e., need 2.75x longer exposure to give equivalent film OD
  - i.e., auto exp time with Tecnai emulsion setting of 2.8 should give OD of ~0.7
- factor of 5.5 for Kodak 4489 developed in 1:2 dilution of D-19
  - i.e., need 5.5x longer exposure to give equivalent film OD
  - i.e., auto exp time with Tecnai emulsion setting of 5.6 should give OD of ~0.7
- People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution
- Kodak relative speeds (from Kodak literature received 1993) - these values were used to derive above factors
  - Kodak electron image plate in D-19 1:2 dil at 20C for 4 min: 70
  - Kodak 4489 in D-19 1:2 dil at 20C for 4 min: 40
  - Kodak SO-163 in D-19 full-strength + 1g/L benzotriazole (antifog) for 2 min: 15
  - Kodak SO-163 in D-19 1:2 dil at 20C for 4 min: 80
  - Kodak SO-163 in D-19 full-strength at 20C for 4 min: 220
- Grain size goes inversely to film speed

Tecnai F20

Recommended Emulsion settings
- 1.0 cryo samples - this will produce film OD = 0.7 for auto exposure time
- data setting at 1.0 - this will produce reasonably dense numbers with full strength D19 developer
- 1.4 plastic sections - this will produce film OD = 1.0 for auto exposure time

Corresponding film OD and specimen dose at 200kV
- Using SO-163 film developed for 12-15 min in full-strength D19
- Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
- film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
- Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
- Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage
- at 100kV, get an increase in film OD of ~1.6 for the same dose

JEOL2100

Faraday cage used by Rob Hynes to check calibration of the beam current measured from the screen
11 pA/cm2 will produce a film OD=1.0 with 1 sec exposure
emulsion setting of 19 will produce film OD=1.0 with auto exposure time
to calculate specimen dose
- 1A = 1Coulomb/sec
- 1pA = 10^-12C/sec = 10^-12 e^-/1.6x10^-19 sec = 6.25x10⁶ e^-/sec
- 10 pA/cm² = 6.25x10⁷e/sec-cm² * 1 um²/10⁸ A² = 0.625 e^-/sec/um²
- at mag, multiply above result by mag²*1 um²/10⁸A²
  - at 50,000x, 10 pA/cm² corresponds to 15.6 e/A²
    - but Jaap claims a 0.8 mag factor to small screen, suggesting that 10 pA/cm² => 10 e/A²
  - at 60,000x, 10 pA/cm² corresponds to 22.5 e/A²
    - but Jaap claims a 0.8 mag factor to small screen, suggesting that 10 pA/cm² => 14.4 e/A²
CCD counts/electron =

JEOL3200

Gatan engineers claim 17.743 counts/electron
- post-column magnification of 1.3
- pA/cm² *11/1000 = nA total current (pA/cm2 measured on small screen)

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 930 Aug 2007 - Main.DavidStokes

META TOPICPARENT	name="TecnaiInstructions"

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM

Taking a film image

Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
Lift screen
Cover glass viewing chamber
Low Dose mode
- Click exposure found in exposure tab
Other viewing modes
- Under camera menu
- Plate camera (user) menu (pullout)
- Settings Tab

Electron Dose and film OD

All microscopes will have emulsion settings to provide OD=0.7 for automatic exposure

John Berriman notes regarding film OD
- exposure with 1 e/um2 ( 16.7 pA/cm2) 12 mins Full-Strength D19 gives
  - 80kV: OD 2.2
  - 120kV: OD 2.0
  - 200kV: OD 1.4
  - 300kV: OD 1.0
  - 400kV: OD 0.7

Film speeds (according to Kodak literature with 80 kV electrons)
- factor of 1.0 for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
- factor of 2.75 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water)
  - i.e., need 2.75x longer exposure to give equivalent film OD
  - i.e., auto exp time with Tecnai emulsion setting of 2.8 should give OD of ~0.7
- factor of 5.5 for Kodak 4489 developed in 1:2 dilution of D-19
  - i.e., need 5.5x longer exposure to give equivalent film OD
  - i.e., auto exp time with Tecnai emulsion setting of 5.6 should give OD of ~0.7
- People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution
- Kodak relative speeds (from Kodak literature received 1993) - these values were used to derive above factors
  - Kodak electron image plate in D-19 1:2 dil at 20C for 4 min: 70
  - Kodak 4489 in D-19 1:2 dil at 20C for 4 min: 40
  - Kodak SO-163 in D-19 full-strength + 1g/L benzotriazole (antifog) for 2 min: 15
  - Kodak SO-163 in D-19 1:2 dil at 20C for 4 min: 80
  - Kodak SO-163 in D-19 full-strength at 20C for 4 min: 220
- Grain size goes inversely to film speed

Tecnai F20

Recommended Emulsion settings
- 1.0 cryo samples - this will produce film OD = 0.7 for auto exposure time
- data setting at 1.0 - this will produce reasonably dense numbers with full strength D19 developer
- 1.4 plastic sections - this will produce film OD = 1.0 for auto exposure time

Corresponding film OD and specimen dose at 200kV
- Using SO-163 film developed for 12-15 min in full-strength D19
- Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
- film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
- Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
- Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage
- at 100kV, get an increase in film OD of ~1.6 for the same dose

JEOL2100

Faraday cage used by Rob Hynes to check calibration of the beam current measured from the screen
11 pA/cm2 will produce a film OD=1.0 with 1 sec exposure
emulsion setting of 19 will produce film OD=1.0 with auto exposure time
to calculate specimen dose
- 1A = 1Coulomb/sec
- 1pA = 10^-12C/sec = 10^-12 e^-/1.6x10^-19 sec = 6.25x10⁶ e^-/sec
- 10 pA/cm² = 6.25x10⁷e/sec-cm² * 1 um²/10⁸ A² = 0.625 e^-/sec/um²
- at mag, multiply above result by mag²*1 um²/10⁸A²
  - at 50,000x, 10 pA/cm² corresponds to 15.6 e/A²
    - but Jaap claims a 0.8 mag factor to small screen, suggesting that 10 pA/cm² => 10 e/A²
  - at 60,000x, 10 pA/cm² corresponds to 22.5 e/A²
    - but Jaap claims a 0.8 mag factor to small screen, suggesting that 10 pA/cm² => 14.4 e/A²

Added:

>
>

CCD counts/electron =

JEOL3200

Gatan engineers claim 17.743 counts/electron
- post-column magnification of 1.3
- pA/cm² *11/1000 = nA total current (pA/cm2 measured on small screen)

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 830 Aug 2007 - Main.DavidStokes

   META TOPICPARENT   name="TecnaiInstructions" 

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM



 Taking a film image 

 

 Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
  Lift screen
  Cover glass viewing chamber
  Low Dose mode 
 Click exposure found in exposure tab
 
  Other viewing modes 
 Under camera menu
  Plate camera (user) menu (pullout)
  Settings Tab
 
 

 Electron Dose and film OD 
 

 All microscopes will have emulsion settings to provide OD=0.7 for automatic exposure
 
 

 John Berriman notes regarding film OD 
 exposure with 1 e/um2  ( 16.7 pA/cm2) 12 mins Full-Strength D19 gives 
 80kV:  OD 2.2
  120kV: OD 2.0
  200kV: OD 1.4
  300kV: OD 1.0
  400kV: OD 0.7
 
 
 
 

 Film speeds (according to Kodak literature with 80 kV electrons) 
 factor of 1.0 for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
  factor of 2.75 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water) 
 i.e., need 2.75x longer exposure to give equivalent film OD
  i.e., auto exp time with Tecnai emulsion setting of 2.8 should give OD of ~0.7
 
  factor of 5.5 for Kodak 4489 developed in 1:2 dilution of D-19 
 i.e., need 5.5x longer exposure to give equivalent film OD
  i.e., auto exp time with Tecnai emulsion setting of 5.6 should give OD of ~0.7
 
  People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution
  Kodak relative speeds (from Kodak literature received 1993) - these values were used to derive above factors 
 Kodak electron image plate in D-19 1:2 dil at 20C for 4 min:  70
  Kodak 4489 in D-19 1:2 dil at 20C for 4 min:  40
  Kodak SO-163 in D-19 full-strength + 1g/L benzotriazole (antifog) for 2 min:  15
  Kodak SO-163 in D-19 1:2 dil at 20C for 4 min:  80
  Kodak SO-163 in D-19 full-strength at 20C for 4 min:  220
 
  Grain size goes inversely to film speed
 
 

 Tecnai F20 
 

 Recommended Emulsion settings  
 1.0 cryo samples - this will produce film OD = 0.7 for auto exposure time
  data setting at 1.0 - this will produce reasonably dense numbers with full strength D19 developer
  1.4 plastic sections - this will produce film OD = 1.0 for auto exposure time
 
 
 

 Corresponding film OD and specimen dose at 200kV 
 Using SO-163 film developed for 12-15 min in full-strength D19
  Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
  film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
  Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
  Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage 
  at 100kV, get an increase in film OD of ~1.6 for the same dose
 
 

 JEOL2100 
 
 Faraday cage used by Rob Hynes to check calibration of the beam current measured from the screen
  11 pA/cm2 will produce a film OD=1.0 with 1 sec exposure
  emulsion setting of 19 will produce film OD=1.0 with auto exposure time
  to calculate specimen dose 
 1A = 1Coulomb/sec
  1pA = 10^-12C/sec = 10^-12 e^-/1.6x10^-19 sec = 6.25x10⁶ e^-/sec
  10 pA/cm² = 6.25x10⁷e/sec-cm² * 1 um²/10⁸ A² = 0.625 e^-/sec/um²
-  META TOPICPARENT
+ name="TecnaiInstructions"
-<
<
+ at 50,000x, multiply above result by 50000²*1 um²/10⁸A²
->
>
+ at mag, multiply above result by mag²*1 um²/10⁸A²
->
>
+ at 50,000x, 10 pA/cm² corresponds to 15.6 e/A² 
 but Jaap claims a 0.8 mag factor to small screen, suggesting that 10 pA/cm² => 10 e/A²
 
  at 60,000x, 10 pA/cm² corresponds to 22.5 e/A² 
 but Jaap claims a 0.8 mag factor to small screen, suggesting that 10 pA/cm² => 14.4 e/A²
 Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM


-- DavidStokes - 18 Mar 2005
 

 Set ALLOWTOPICCHANGE = CemfacGroup

Revision 730 Aug 2007 - Main.DavidStokes

   META TOPICPARENT   name="TecnaiInstructions" 

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM



 Taking a film image 

 

 Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
  Lift screen
  Cover glass viewing chamber
  Low Dose mode 
 Click exposure found in exposure tab
 
  Other viewing modes 
 Under camera menu
  Plate camera (user) menu (pullout)
  Settings Tab
 
 

 Electron Dose and film OD
-  META TOPICPARENT
+ name="TecnaiInstructions"
-<
<
+ Recommended Emulsion settings
->
>
+ All microscopes will have emulsion settings to provide OD=0.7 for automatic exposure
-<
<
+.4 plastic sections 
  1.0 cryo samples
  data setting at 1.0
-<
<
+ Corresponding film OD and specimen dose at 200kV 
 Using SO-163 film developed for 12-15 min in full-strength D19
  Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
  film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
  Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
  Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage 
  at 100kV, get an increase in film OD of ~1.6 for the same dose
->
>
+ John Berriman notes regarding film OD 
 exposure with 1 e/um2  ( 16.7 pA/cm2) 12 mins Full-Strength D19 gives 
 80kV:  OD 2.2
  120kV: OD 2.0
  200kV: OD 1.4
  300kV: OD 1.0
  400kV: OD 0.7
  Film speeds (according to Kodak literature with 80 kV electrons)
-<
<
+ above data for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
->
>
+ factor of 1.0 for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
  factor of 2.75 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water)
-<
<
+ i.e., auto exp time with emulsion setting of 2.8 should give OD of ~0.7
->
>
+ i.e., need 2.75x longer exposure to give equivalent film OD
->
>
+ i.e., auto exp time with Tecnai emulsion setting of 2.8 should give OD of ~0.7
  factor of 5.5 for Kodak 4489 developed in 1:2 dilution of D-19
-<
<
+ i.e., auto exp time with emulsion setting of 5.6 should give OD of ~0.7
->
>
+ i.e., need 5.5x longer exposure to give equivalent film OD
->
>
+ i.e., auto exp time with Tecnai emulsion setting of 5.6 should give OD of ~0.7
  People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution
-<
<
+ Kodak relative speeds (from Kodak literature received 1993)
->
>
+ Kodak relative speeds (from Kodak literature received 1993) - these values were used to derive above factors
  Kodak electron image plate in D-19 1:2 dil at 20C for 4 min:  70
  Kodak 4489 in D-19 1:2 dil at 20C for 4 min:  40
  Kodak SO-163 in D-19 full-strength + 1g/L benzotriazole (antifog) for 2 min:  15
  Kodak SO-163 in D-19 1:2 dil at 20C for 4 min:  80
  Kodak SO-163 in D-19 full-strength at 20C for 4 min:  220
 
  Grain size goes inversely to film speed
-<
<
+ John Berriman notes regarding film OD 
 exposure with 1 e/um2  ( 16.7 pA/cm2) 12 mins Full-Strength D19 gives 
 80kV:  OD 2.2
  120kV: OD 2.0
  200kV: OD 1.4
  300kV: OD 1.0
  400kV: OD 0.7
->
>
+ Tecnai F20 
 

 Recommended Emulsion settings  
 1.0 cryo samples - this will produce film OD = 0.7 for auto exposure time
  data setting at 1.0 - this will produce reasonably dense numbers with full strength D19 developer
  1.4 plastic sections - this will produce film OD = 1.0 for auto exposure time
->
>
+ Corresponding film OD and specimen dose at 200kV 
 Using SO-163 film developed for 12-15 min in full-strength D19
  Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
  film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
  Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
  Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage 
  at 100kV, get an increase in film OD of ~1.6 for the same dose
 
 

 JEOL2100 
 
 Faraday cage used by Rob Hynes to check calibration of the beam current measured from the screen
  11 pA/cm2 will produce a film OD=1.0 with 1 sec exposure
  emulsion setting of 19 will produce film OD=1.0 with auto exposure time
  to calculate specimen dose 
 1A = 1Coulomb/sec
  1pA = 10^-12C/sec = 10^-12 e^-/1.6x10^-19 sec = 6.25x10⁶ e^-/sec
  10 pA/cm² = 6.25x10⁷e/sec-cm² * 1 um²/10⁸ A² = 0.625 e^-/sec/um²
  at 50,000x, multiply above result by 50000²*1 um²/10⁸A²
 Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM


-- DavidStokes - 18 Mar 2005
 

 Set ALLOWTOPICCHANGE = CemfacGroup

Revision 607 Dec 2006 - Main.DavidStokes

   META TOPICPARENT   name="TecnaiInstructions" 

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM



 Taking a film image
-  META TOPICPARENT
+ name="TecnaiInstructions"
-<
<
+ Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
  Lift screen
  Cover glass viewing chamber
  Low Dose mode 
 Click exposure found in exposure tab
 
  Other viewing modes 
 Under camera menu
  Plate camera (user) menu (pullout)
  Settings Tab
->
>
+ Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
  Lift screen
  Cover glass viewing chamber
  Low Dose mode 
 Click exposure found in exposure tab
 
  Other viewing modes 
 Under camera menu
  Plate camera (user) menu (pullout)
  Settings Tab
  Electron Dose and film OD
-<
<
+ Recommended Emulsion settings  
 1.4 plastic sections 
  1.0 cryo samples
  data setting at 1.0
->
>
+ Recommended Emulsion settings  
 1.4 plastic sections 
  1.0 cryo samples
  data setting at 1.0
-<
<
+ Corresponding film OD and specimen dose at 200kV 
 Using SO-163 film developed for 12-15 min in full-strength D19
  Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
  film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
  Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
  Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage 
  at 100kV, get an increase in film OD of ~1.6 for the same dose
->
>
+ Corresponding film OD and specimen dose at 200kV 
 Using SO-163 film developed for 12-15 min in full-strength D19
  Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
  film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
  Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
  Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage 
  at 100kV, get an increase in film OD of ~1.6 for the same dose
-<
<
+ Film speeds (according to Kodak literature with 80 kV electrons) 
 above data for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
  factor of 2.75 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water) 
 i.e., auto exp time with emulsion setting of 2.8 should give OD of ~0.7
 
  factor of 5.5 for Kodak 4489 developed in 1:2 dilution of D-19 
 i.e., auto exp time with emulsion setting of 5.6 should give OD of ~0.7
 
  People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution
  Kodak relative speeds (from Kodak literature received 1993) 
 Kodak electron image plate in D-19 1:2 dil at 20C for 4 min:  70
  Kodak 4489 in D-19 1:2 dil at 20C for 4 min:  40
  Kodak SO-163 in D-19 full-strength + 1g/L benzotriazole (antifog) for 2 min:  15
  Kodak SO-163 in D-19 1:2 dil at 20C for 4 min:  80
  Kodak SO-163 in D-19 full-strength at 20C for 4 min:  220
 
  Grain size goes inversely to film speed
->
>
+ Film speeds (according to Kodak literature with 80 kV electrons) 
 above data for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
  factor of 2.75 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water) 
 i.e., auto exp time with emulsion setting of 2.8 should give OD of ~0.7
 
  factor of 5.5 for Kodak 4489 developed in 1:2 dilution of D-19 
 i.e., auto exp time with emulsion setting of 5.6 should give OD of ~0.7
 
  People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution
  Kodak relative speeds (from Kodak literature received 1993) 
 Kodak electron image plate in D-19 1:2 dil at 20C for 4 min:  70
  Kodak 4489 in D-19 1:2 dil at 20C for 4 min:  40
  Kodak SO-163 in D-19 full-strength + 1g/L benzotriazole (antifog) for 2 min:  15
  Kodak SO-163 in D-19 1:2 dil at 20C for 4 min:  80
  Kodak SO-163 in D-19 full-strength at 20C for 4 min:  220
 
  Grain size goes inversely to film speed
->
>
+ John Berriman notes regarding film OD 
 exposure with 1 e/um2  ( 16.7 pA/cm2) 12 mins Full-Strength D19 gives 
 80kV:  OD 2.2
  120kV: OD 2.0
  200kV: OD 1.4
  300kV: OD 1.0
  400kV: OD 0.7
 Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM


-- DavidStokes - 18 Mar 2005
-<
<
+ Set ALLOWTOPICCHANGE = CemfacGroup
->
>
+ Set ALLOWTOPICCHANGE = CemfacGroup
-<
<

Revision 505 May 2006 - Main.DavidStokes

META TOPICPARENT	name="TecnaiInstructions"

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM

Taking a film image

Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
Lift screen
Cover glass viewing chamber
Low Dose mode
- Click exposure found in exposure tab
Other viewing modes
- Under camera menu
- Plate camera (user) menu (pullout)
- Settings Tab

Electron Dose and film OD

Recommended Emulsion settings
- 1.4 plastic sections
- 1.0 cryo samples
- data setting at 1.0

Corresponding film OD and specimen dose at 200kV
- Using SO-163 film developed for 12-15 min in full-strength D19
- Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
- film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
- Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
- Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage
- at 100kV, get an increase in film OD of ~1.6 for the same dose

Film speeds (according to Kodak literature with 80 kV electrons)
- above data for Kodak SO-163 developed 12-15 min at full-strength D-19 developer

Changed:

<
<

- factor of 2.44 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water)
  - i.e., auto exp time with emulsion setting of 2.8 should give OD of ~0.8
- factor of 4.88 for Kodak 4489 developed in 1:2 dilution of D-19
  - i.e., auto exp time with emulsion setting of 5.6 should give OD of ~0.8

>
>

- factor of 2.75 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water)
  - i.e., auto exp time with emulsion setting of 2.8 should give OD of ~0.7
- factor of 5.5 for Kodak 4489 developed in 1:2 dilution of D-19
  - i.e., auto exp time with emulsion setting of 5.6 should give OD of ~0.7

- People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution

Changed:

<
<

- Kodak relative speeds
  - Kodak electron image plate in D-19 1:2 dil at 20C for 4 min: 100
  - Kodak 4489 in D-19 1:2 dil at 20C for 4 min: 45
  - Kodak SO-163 in D-19 full-strength + 1g/L Kodak Anti-Fog No. 1 for 2 min: 40
  - Kodak SO-163 in D-19 1:2 dil at 20C for 4 min: 90

>
>

- Kodak relative speeds (from Kodak literature received 1993)
  - Kodak electron image plate in D-19 1:2 dil at 20C for 4 min: 70
  - Kodak 4489 in D-19 1:2 dil at 20C for 4 min: 40
  - Kodak SO-163 in D-19 full-strength + 1g/L benzotriazole (antifog) for 2 min: 15
  - Kodak SO-163 in D-19 1:2 dil at 20C for 4 min: 80

- - Kodak SO-163 in D-19 full-strength at 20C for 4 min: 220
- Grain size goes inversely to film speed

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 405 May 2006 - Main.DavidStokes

META TOPICPARENT	name="TecnaiInstructions"

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM

Taking a film image

Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
Lift screen
Cover glass viewing chamber
Low Dose mode
- Click exposure found in exposure tab
Other viewing modes
- Under camera menu
- Plate camera (user) menu (pullout)
- Settings Tab

Deleted:

<
<

Emulsion density (1.4 plastic sections- 1.0 cryo sections)
- Emulsion setting generally at 1.0 and Data setting at 1.0
- This should produce a film OD of 0.7 using the automatic exposure time
- film OD of 0.7 corresponds to 0.5 electrons/um², which at 50kx corresponds to 12.5 electrons/A²

Changed:

<
<

Record date and ending plate number in online logbook (Grey Matter) accessible from web browser on TEM data computer

>
>

Electron Dose and film OD

Added:

>
>

Recommended Emulsion settings
- 1.4 plastic sections
- 1.0 cryo samples
- data setting at 1.0

Corresponding film OD and specimen dose at 200kV
- Using SO-163 film developed for 12-15 min in full-strength D19
- Automatic exposure times using emulsion setting of 1.0 will produce a film OD of 0.7
- film OD of 0.7 corresponds to 0.5 electrons/um2, which at 50kx corresponds to 12.5 electrons/A2
- Automatic exposure times using emulsion setting of 1.4 will produce a film OD of 1.0
- Automatic exposure times using emulsion setting of 2.0 will produce film OD of 1.4, with corresponding increase in specimen dosage
- at 100kV, get an increase in film OD of ~1.6 for the same dose

Film speeds (according to Kodak literature with 80 kV electrons)
- above data for Kodak SO-163 developed 12-15 min at full-strength D-19 developer
- factor of 2.44 for developing in 1:2 dilution of D-19 for 4 min (1 part devloper, 2 parts water)
  - i.e., auto exp time with emulsion setting of 2.8 should give OD of ~0.8
- factor of 4.88 for Kodak 4489 developed in 1:2 dilution of D-19
  - i.e., auto exp time with emulsion setting of 5.6 should give OD of ~0.8
- People often use 1:1 dilution for 4 min, which should produce somewhat darker films than 1:2 dilution
- Kodak relative speeds
  - Kodak electron image plate in D-19 1:2 dil at 20C for 4 min: 100
  - Kodak 4489 in D-19 1:2 dil at 20C for 4 min: 45
  - Kodak SO-163 in D-19 full-strength + 1g/L Kodak Anti-Fog No. 1 for 2 min: 40
  - Kodak SO-163 in D-19 1:2 dil at 20C for 4 min: 90
  - Kodak SO-163 in D-19 full-strength at 20C for 4 min: 220
- Grain size goes inversely to film speed

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 304 May 2006 - Main.DavidStokes

   META TOPICPARENT   name="TecnaiInstructions"
-  META TOPICPARENT
+ name="TecnaiInstructions"
-<
<
+Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM



 Taking a film image 

 

 Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
  Lift screen
  Cover glass viewing chamber
  Low Dose mode 
 Click exposure found in exposure tab
 
  Other viewing modes 
 Under camera menu
  Plate camera (user) menu (pullout)
  Settings Tab
 
  Emulsion density (1.4 plastic sections- 1.0 cryo sections)
 
 

 Record date and ending plate number in online logbook (Grey Matter) accessible from web browser on TEM data computer
 




Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM


-- DavidStokes - 18 Mar 2005
 

 Set ALLOWTOPICCHANGE = CemfacGroup
->
>
+Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM



 Taking a film image 

 

 Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
  Lift screen
  Cover glass viewing chamber
  Low Dose mode 
 Click exposure found in exposure tab
 
  Other viewing modes 
 Under camera menu
  Plate camera (user) menu (pullout)
  Settings Tab
 
  Emulsion density (1.4 plastic sections- 1.0 cryo sections) 
 Emulsion setting generally at 1.0 and Data setting at 1.0
  This should produce a film OD of 0.7 using the automatic exposure time
  film OD of 0.7 corresponds to 0.5 electrons/um², which at 50kx corresponds to 12.5 electrons/A²
 
 
 

 Record date and ending plate number in online logbook (Grey Matter) accessible from web browser on TEM data computer
 




Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM


-- DavidStokes - 18 Mar 2005
->
>
+ Set ALLOWTOPICCHANGE = CemfacGroup

Revision 222 Mar 2005 - Main.DavidStokes

META TOPICPARENT	name="TecnaiInstructions"

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM

Taking a film image

Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
Lift screen
Cover glass viewing chamber
Low Dose mode
- Click exposure found in exposure tab
Other viewing modes
- Under camera menu
- Plate camera (user) menu (pullout)
- Settings Tab
Emulsion density (1.4 plastic sections- 1.0 cryo sections)

Record date and ending plate number in online logbook (Grey Matter) accessible from web browser on TEM data computer

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Added:

>
>

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 118 Mar 2005 - Main.DavidStokes

META TOPICPARENT	name="TecnaiInstructions"

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM

Taking a film image

Insert small screen adjust brightness until the exposure meter reads 1.0 sec (bottom left)
Lift screen
Cover glass viewing chamber
Low Dose mode
- Click exposure found in exposure tab
Other viewing modes
- Under camera menu
- Plate camera (user) menu (pullout)
- Settings Tab
Emulsion density (1.4 plastic sections- 1.0 cryo sections)

Record date and ending plate number in online logbook (Grey Matter) accessible from web browser on TEM data computer

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

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