Difference: IntegrativeApproaches ( vs. 1)
Revision 115 Mar 2006 - Main.DavidCowburn
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Integrative approached to multidomain protein structure.[1] Using standard NMR methods to compare individual and multiple domains.Gosser, Yuying Q.; Zheng, Jie; Overduin, Michael; Mayer, Bruce J., Cowburn, D. “The solution structure of Abl SH3, and its relationship to SH2 in the SH(32) construct.” (1995) Structure, 3, 1075-1086.[2] Segmental labeling.Xu, Rong; Ayers, Brenda; Cowburn, David; Muir, Tom W. (1999) "Chemical Ligation of Folded Recombinant Proteins: Segmental Isotopic Labeling of Domains for NMR Studies" PNAS, 96, 388-393. Cowburn D, Muir TW Segmental isotopic labeling using expressed protein ligation METHODS IN ENZYMOLOGY 339: 41-54 Part B 2001[3] NMR developments related to sequential labeling.Camarero, J. A., Shekhtman, A., Campbell, E. A., Chlenov, M., Gruber, T. M., Bryant, D. A., Darst, S. A., Cowburn, D., and Muir, T. W. (2002). “Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR”. Proceedings of the National Academy of Sciences of the United States of America 99, 8536-8541. Romanelli, A., Shekhtman, A., Cowburn, D., and Muir, T. W. (2004). “Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction”. Proceedings of the National Academy of Sciences U S A 101, 6397-6402 UNITED STATES PROVISONAL PATENT APPLICATION for COWBURN, et al., for METHODS AND DEVICES FOR CHARACTERIZING MACROMOLECULAR COMPLEXES USING ISOTOPE LABELING TECHNIQUES, U.S. Serial No. 60,650,725, Filed February 7, 2005[4] Domain orientation determined by NMR.Fushman, David; Xu, Rong; Cowburn, David. (1999) "Direct determination of changes of interdomains orientation on ligation: use of the orientational dependence of 15N NMR relaxation in Abl SH(32)" Biochemistry, 38, 10225-10230. Fushman, David; Ghose, Ranajeet; Cowburn, David (2000) "The effect of finite sampling on the determination of orientational properties: A theoretical treatment with application to interatomic vectors in proteins", J. Am. Chem. Soc., 122, 10640-10649.[5] Bivalent ligands to probe multi domain systems.Cowburn, David; Zheng, Jie; Xu, Qinghong; Barany, George. “Enhanced affinities and specificities of consolidated ligands for the SH3 and SH2 domains of Abelson protein tyrosine kinase”. (1995) J. Biol. Chem., 270, 26738-26741. Xu, Qinghong; Zheng, Jie; Xu, Rong; Barany, George; Cowburn, David. (1999) "Flexibility of Interdomain Contacts Revealed by Topological Isomers of Bivalent Consolidated Ligands: Abelson SH(32)" Biochemistry, 38, 3491-7. Xu, Rong; Cahill, Sean; Cowburn, David. (1999) "Triple resonance-based assignment for Abl SH(32) and its complex with a consolidated ligand" J. Biomol. NMR, 4, 187-8. -- DavidCowburn - 15 Mar 2006 |
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