Difference: NewProposal0911111020 (1 vs. 4)

* Set ALLOWTOPICVIEW = DavidCowburn, DirectorGroup, NysbcadminGroup

Dear Dr. Gupta:

Thanks for your inquiry. We probably should discuss your needs in some detail because the size of the dimer you have is pretty much at the limit of what NMR can effectively help you with. So getting some useful results for you may be quite lengthy both in terms of NMR and of sample preparation. Of course, a quick survey of what you have know is entirely practical and can be rapidly arranged. Have you run your current samples on the RU 600 system?

Any substantial allocations of NMR time for RU at NYSBC are under the review of Prof. Muir and Dr. Yufeng Wei , and will be coordinated with them.

You are a registered NYSBC intranet user, and it may be helpful to you to look at http://www.nysbc.net/twiki/bin/view/Main/NmrfacFAQs and http://www.nysbc.net/twiki/bin/view/Main/NmrfacGroup

Please address your replies to nmrschedule@nysbc.org, so that all the people involved can look at your project and try to help move it along. I'd suggest we include Prof. Sakmar in some discussion so that the scale of what's needed can be properly understood by all concerned.

David Cowburn

Reply-To:nmrschedule@nysbc.org

On Tue, Nov 10, 2009 at 10:54 PM, David Stokes <stokes@nyu.edu> wrote: Ruchi, I am forwarding your request to the NMR group at NYSBC. David

ruchi gupta wrote: Dear Prof. Stokes I am Ruchi writing from Rockefeller university from the laboratory of Prof. Thomas P. Sakmar. I am writing to request for some NMR time preferably on the cryo-probe. We have a 50kDa protein at hand which exists as a dimer. For over 2 years, we have been trying to crystallize this protein in vain. Our initial MALS studies have shown this protein to be very elongated (Rh 6.2nm) and it seems flexible. Thus we wanted to collect some initial NMR data to get an idea of how unstructed the protein might be and if possible which regions are most unstructured. We understand that solving the whole structure by NMR might not be feasible but I think we can definitely get some preliminary idea of the structural features of the protein with your kind assistance. We have purified both N15 labelled and unlabelled forms of the protein to homogenity. We would like to proceed to collect some NMR data on the same. Both me and my colleague Neeraj Kapoor dont have much experience on NMR except 1D NMR. Thus we request you for your guidance and suggestions in the process. Please let us know if you can kindly meet us and guide us towards collecting some initial data. I would be glad to answer any further questions on the structural project at hand. Thanking you With Regards Ruchi

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