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Workshop in Dual-Beam Scanning Electron Microscopy at the New York Structural Biology Center

This workshop will introduce the new Helios 650 Nanolab microscope at NYSBC and illustrate its capabilities for producing large scale (10um x 10um x 10um) 3D reconstructions through resin-embedded tissue as well as for preparing frozen, whole-mount cells for electron tomography. In the literature, this method is alternately referred to as Focused Ion Beam (FIB) milling and Ion Abrasion SEM.

• January 26, 2012
• 1-5:30 pm
• NYSBC Seminar Room A-11
• To register, please RSVP to cem@nysbc.org

Agenda

• 1:00 pm - David Stokes/Bill Rice (NYSBC): Introduction to the Dual-Beam SEM Microscope
• 1:45 pm - Dave Hall (Einstein College of Medicine): Special Sample Procedures for C. Elegans; Enhanced en bloc Staining
• 2:30 coffee
• 3:00 - Gavin Murphy (NCI/NIH): Methods for Reconstructing HIV-infected T cells: Correlative LIght & FIB-SEM Microscopy and Image Processing
• 3:45 - Peijun Zhang (Univ. of Pittsburgh): Cryo-FIB and correlative approaches for 3D imaging of native cells
• 4:30 - Paul Anzalone (FEI Applications Specialist) tour/demo of dual-beam microscope and reconstruction software
• 5:30 - Reception