Difference: NmredNotes (1 vs. 2)

Notes from a recent EU meeting on training NMR students.

How to meet these challenges

The Young Researchers must be immersed in laboratories where broad know-how has been acquired

(1) Molecular biology

Requires well-equipped laboratories such as are available in Florence, Frankfurt, Stockholm and Utrecht.

(2) Elementary theory of NMR spectroscopy

Requires good teachers and structured courses.

(3) Basic practice of NMR spectroscopy

Requires modem spectrometers and experienced supervisors.

(4) Interpretation of NMR spectra

Requires experienced supervisors,

(5) Molecular Dynamics (MD)

Requires experienced supervisors and modern computers

(6) Advanced theory of NMR spectroscopy

Requires outstanding teachers.

(7) Advanced spectroscopy

Requires highly-skilled experimentalists.

Some thoughts about training in the field of Biomolecular NMR

This field lies at the intersection of three major areas :

C-24 Instrumental Techniques, Analysis and Sensors

C-25 Computational Chemistry and Modeling

L-21 Molecular Biophysics

Challenges

The Young Researchers must learn to master a great deal of know-how :

(1) Molecular biology Handling of plasmids, bacterial growth with isotopically enriched media, protein purification, handling of NMR samples, control of sample precipitation and aggregation.

(2) Elementary theory of NMR spectroscopy Concepts such as magnetization, relaxation, Fourier transformation, etc. Basic quantum mechanics : the density operator and its evolution.

(3) Basic practice of NMR spectroscopy How to excite the magnetization, observe signals, carry out Fourier transformations in one and two dimensions, etc.

(4) Interpretation of NMR spectra How to assign resonances in NMR spectra. How to transform information about internuclear distances (Overhauser effects) into a model of the molecular structure.

(5) Molecular Dynamics (MD) Computation of internal motions of biomolecules and energy minimization.

(6) Advanced theory of NMR spectroscopy More quantum mechanics : Liouville operators. Redfield’s relaxation theory. Paramagnetic systems. Correlated fluctuations. Residual Dipolar Couplings (RDC’s).

(7) Advanced spectroscopy Accurate measurement of cross-correlation effects. Control of systematic errors.

-- DavidCowburn - 07 Sep 2004

Notes from a recent EU meeting on training NMR students.

How to meet these challenges

The Young Researchers must be immersed in laboratories where broad know-how has been acquired

(1) Molecular biology

Requires well-equipped laboratories such as are available in Florence, Frankfurt, Stockholm and Utrecht.

(2) Elementary theory of NMR spectroscopy

Requires good teachers and structured courses.

(3) Basic practice of NMR spectroscopy

Requires modem spectrometers and experienced supervisors.

(4) Interpretation of NMR spectra

Requires experienced supervisors,

(5) Molecular Dynamics (MD)

Requires experienced supervisors and modern computers

(6) Advanced theory of NMR spectroscopy

Requires outstanding teachers.

(7) Advanced spectroscopy

Requires highly-skilled experimentalists.

Some thoughts about training in the field of Biomolecular NMR

This field lies at the intersection of three major areas :

C-24 Instrumental Techniques, Analysis and Sensors

C-25 Computational Chemistry and Modeling

L-21 Molecular Biophysics

Challenges

The Young Researchers must learn to master a great deal of know-how :

(1) Molecular biology Handling of plasmids, bacterial growth with isotopically enriched media, protein purification, handling of NMR samples, control of sample precipitation and aggregation.

(2) Elementary theory of NMR spectroscopy Concepts such as magnetization, relaxation, Fourier transformation, etc. Basic quantum mechanics : the density operator and its evolution.

(3) Basic practice of NMR spectroscopy How to excite the magnetization, observe signals, carry out Fourier transformations in one and two dimensions, etc.

(4) Interpretation of NMR spectra How to assign resonances in NMR spectra. How to transform information about internuclear distances (Overhauser effects) into a model of the molecular structure.

(5) Molecular Dynamics (MD) Computation of internal motions of biomolecules and energy minimization.

(6) Advanced theory of NMR spectroscopy More quantum mechanics : Liouville operators. Redfield’s relaxation theory. Paramagnetic systems. Correlated fluctuations. Residual Dipolar Couplings (RDC’s).

(7) Advanced spectroscopy Accurate measurement of cross-correlation effects. Control of systematic errors.

-- DavidCowburn - 07 Sep 2004