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CEM Staff Emergency Contact Name Cell Phone After Hours Email SMS? (Y/N) Bill Rice 917 528 0333 rice@nysbc.org Y David Stokes 646 284 8775 ...
Contents IF CCD blank enable window is closed, then CCD blanking box won't work Occasionally, box won't work even though CCD blank Enable is open and checked ...
Contents 2013 CEM webpage refresh Background Refresh of CEM webpage to accommodate NYSBC new template. Architecture Public CemTwikiToWebList CemTwikiToWebMainPageTest ...
Cryo electron Microscopy (CEM) uses electrons accelerated to almost the speed of light to reveal images of individual molecules and molecular assemblies. The electrons ...
Procedure for using the CEM facility at the NYSBC The NYSBC The New York Structural Biology Center is a 501 (c)(3) corporation incorporated in the State of New York ...
Image processing ZeissDensitometer Z/I Imaging (Zeiss) Photoscan Scanner Data processing computer cluster Users have access to a number ...
Member institution core electron microscopy facilities Albert Einstein College of Medicine of Yeshiva University City University of New York Columbia ...
CEM Administration Contacts CEMStaffEmergencyContact EmergencyCall Staff Procedures Ordering, Shipping and Receiving NYSBC Procedures ...
Contents NEW DUAL BEAM FIB/SEM MICROSCOPE AVAILABLE FOR YOUR USE The New York Structural Biology Center (NYSBC), to which your institution belongs, has recently acquired ...
NEW DUAL BEAM FIB/SEM MICROSCOPE AVAILABLE FOR YOUR USE The New York Structural Biology Center (NYSBC), to which your institution belongs, has recently acquired ...
Contents Annual CEM BBQ 22 August 2014 CEM User meeting and Annual BBQ cembbq2014.pdf: 2014 flier Event: CEM user meeting Location: NYSBC, Room A 11 Time ...
Contents The CLEM workflow is still under development. Sample workflow Cartoon workflow Procedure Instruments Nikon Light Microscope FEI Helios ...
Contents Ed's Overview of EM (cartoon version) Choice of an appropriate structural biological technique Before beginning a project one must match the biological ...
Contact us by e mail: cem@nysbc.org. Mailing address: New York Structural Biology Center CEM Facility 89 Convent Ave New York, NY 10027 USA Phone ...
Contact us: Please send all general inquiries to: cem@nysbc.org. Location: New York Structural Biology Center: 89 Convent Ave, New York, NY 10027 Phone: (212) 939 ...
Contact us: Please send all general inquiries to: cem@nysbc.org. Location: New York Structural Biology Center: 89 Convent Ave, New York, NY 10027 Phone: (212) 939 ...
CEM Course Group Web Site for Graduate Course "CryoElectron Microscopy of Macromolecular Complexes" CemCourseAnnouncement CemCourseAnnouncement14 CemCourseSyllabus14 ...
Contents Dec 10 Molecular Fitting Wily Wriggers Lecture nysbc13.ppt: powerpoint of molecular fitting lecture wriggers.mp3: audio of molecular ...
Contents Player required for arf video files nbr2player.msi: MS windows player for arf files Lectures Structure Validation and Fitting Dec. 9 Lecture ...
Overview Detailed Syllabus: Jan 11 : Introduction SEMC tour Welcome to the Simons Electron Microscopy Center and explanation of the course. A tour of NYSBC and ...
Attendees name position inst lab email credit reg card disc Ana Asenjo tech AECOM Sosa Lab aasenjo@aecom.yu.edu ...
Students name inst lab email position credit disc paper Yi ying Chou MSSM Palese Yi ying.Chou@mssm.edu grad student (2) ...
Contents List of Samples Carbon Film Gold TMV : single particle helical reconstruction phi6 (Paul Gottlieb) : single particle, icosahedral reconstruction ...
Principles Background of structural biology and TEM technique TEM X ray crystallography NMR resolution 2 150 Å 1 5 Å 1 5 Å ...
Electron Crystallography Projects Zinc Transport YiiP protein – structural basis for an alternating access model Micrograph of negatively stained YiiP ...
Electron Tomography Projects Influenza: indentifying structural characteristics in egg adapted vaccine preparations A 7Å thick section through a tomographic ...
Contents Name of the topic Sublevel topic subsub level topic https://filesender.internet2.edu/?vid 4fb117fb 09da 79e8 3afc 0000459e2125 Set ALLOWTOPICVIEW ...
Contents Fib/SEM CemFibSEMProcessing tif2mrc Image .tif rawstack.mrc xfalign matt 0.1 reduce 1 bilinear params 2 sobel rawstack.mrc rawstack.xf xftoxg n 0 rawstack ...
Contents Projects NYU Ned Seeman June 29, 2012 Initial meeting Steve Hubbard/Todd Miller February 23, 2012 First email from Steve March 9, 2012 ...
Microscopes The microscopes at the NYSBC are located in specially built rooms, each one including a concrete slab that is supported directly by the bedrock of the ...
The NYSBC houses a state of the art electron microscopy facility in upper Manhattan. For an overview of the resources in our CEM facility follow the links below. ...
Microscopes The microscopes at the NYSBC are located in specially built rooms, each one including a concrete slab that is supported directly by the bedrock of the ...
Microscopes The microscopes at the NYSBC are located in specially built rooms, each one including a concrete slab that is supported directly by the bedrock of the ...
Cryoelectron Microscopy of Macromolecular Assemblies 12/2/2014: Helical reconstruction Time: 4:00 6:00 pm Instructor: Hernando Sosa (Albert Einstein College of ...
Cryoelectron Microscopy of Macromolecular Assemblies 2/19/2013: Seminar by Co recipient of the 2012 Chemistry Nobel Prize Dr. Brian Kobilka, M.D. (Stanford ...
5/9/2012: Advances in Fluorescence Microscopy of the Cell Please join us for a second single molecule workshop at the New York Structural Biology Center. Time: 9 ...
Previous Staff Members Scientist: Mariena Silvestry Ramos Technical Director: Ruben Diaz Avalos ...
Capabilities of NYSBC's CEM Lab Printable Brochure General Information The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate ...
Capabilities of NYSBC's CEM Lab General Information The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate the intermolecular interactions ...
Helical Reconstruction Projects Name of the topic Set ALLOWTOPICVIEW
Beginning an electron microscopy project High Purity? Usually, a project aimed to determine structural information of a given material is ripe for study when a high ...
Beginning an electron microscopy project High Purity? Usually, a project aimed to determine structural information of a given material is ripe for study when a high ...
Contents 5/12/15: Updated list on redmine Sublevel topic subsub level topic Dewar Slot User Institution Date Notes Contact 1 1 ...
CEMFacGroup Page CEM Administration Page CemChillerWaterLevels CEM Equipment Maintenance Maintenance Checklist Monthly Task Subtask Location ...
Contents Maintenance Checklist 2013 Task Subtask Location Category Date(1) Date(2) Date(3) Date(4) Date(5) Date(6) Date(7) Date ...
CEMFacGroup Page CEM Administration Page 2014 Archive of CemMaintenance 2013 Archive of CemMaintenance CEM Equipment Mainenance Maintenance Checklist Monthly ...
Contents Calendar Standard Institutional Abbreviations: AECO CUNY COLU NYU NYSB MSSM MSKC RU WADS WEIL 30 May 2016 Memorial ...
Contents CemMicroscopeTrainingArchive CEM microscope training sessions Open Training Days are microscope training sessions required before you may sign up for ...
Contents CEM microscope training sessions When there is a large enough group of new users CEM staff will hold a training/re training session. This training session ...
Microscopes The microscopes at the NYSBC are located in specially built rooms, each one including a concrete slab that is supported directly by the bedrock of the ...
Contents General Questions Question: I have a protein and want to do EM, what do I do? If you have a protein or a protein complex that has a MW ~300kDa ...
Contents Names 2dx@nysbc.org, park@nysbc.org, adam.schaffner@mssm.edu, aditidas@illinois.edu, adrianna.trusiak@gmail.com, agrawal@wadsworth.org, akatz@sci.ccny.cuny ...
Contents Nikon Ti U Description Users have access to a Nikon Ti U inverted fluorescence microscope with a FEI Cryo Stage2 system. It allows screening of electron ...
Visit the !CryoEM web site at http://www.nysbc.org/facilities/CEM Cryo EM Operations Committee Members Your representative from the !CryoEM Operations Committee ...
Visit the !CryoEM web site at http://www.nysbc.org/facilities/CEM Cryo EM Operations Committee Members Your representative from the !CryoEM Operations Committee ...
Capabilities of NYSBC's CEM Lab General Information The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate the intermolecular interactions ...
Plasma Cleaning (aka glow discharging) Plasma cleaning is a crucial albeit largely dismissed as irrelevant step in sample preparation of protein samples for electron ...
Plunge freezing With the advent of improved equipment from several different vendors, the preparation of unstained specimens for electron microscopy has become somewhat ...
Contents Signup list: Please add your name to the list Practical session I from Nov. 4 8, 11AM 2PM Topics include programs to process data for: ...
Principles and Protocols for EM structural studies Electron microscopy techniques Structural biology using Transmission Electron Microscopy (TEM) Electron ...
Principles and Protocols for EM structural studies Electron microscopy techniques Structural biology using Transmission Electron Microscopy (TEM) Electron ...
Published work using the NYSBC Cryo EM Facility. Examples of work done at the NYSBC List of Publications Examples of work done at the NYSBC ...
go to CemPublications
Current NYSBC Research Projects Collaborations Electron crystallography Helical reconstruction Single particle analysis ...
Contents See general guidelines for metal shadowing here. Rotary shadowing using the Edwards Auto 306 Evaporator General setup Make sure gloves are worn at ...
Sample preparation In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology ...
Contents Signup for sample prep equipment Rules signup on a first come first served basis signup with your !TwikiName list the time period you want ...
Sample preparation In addition to the state of the art electron microscopes, the NYSBC is fully equipped to meet the sample preparation needs of the structural biology ...
Overview of EM Scheduling at NYSBC Time on our 200kV electron microscopes is allocated per institution according the calendar shown on CemMicroscopeSchedule. Each ...
Courses and Seminars related to !CryoEM At the NYSBC CEM User meeting and Annual BBQ Event: CEM user meeting Location: NYSBC, Room A 11 Time: Friday, August 22, ...
(Archive)Courses and Seminars related to !CryoEM 2013 At the NYSBC Semester long Course : "Cryoelectron Microscopy of Macromolecular Assemblies" This comprehensive ...
Contents Ongoing issues Tecnai New apertures installed March 6 2015 Objective: 100 um, 50 um, 50 um, 40 um condenser: 150 um, 100 um, 70 um, 50 um BUT third ...
Procedure for using the CEM facility at the NYSBC The NYSBC The New York Structural Biology Center is a 501 (c)(3) corporation incorporated in the State of New York ...
Single Particle Projects Random Conical Tilt (RCT) procedure methods used Oct 2012 Collect Data Prepare grids typically negative stain Use SerialEM to ...
Overview Interested in Cryoem? (How to begin a project) CemInterested Cryoem at NYSBC CemIntroduction General info on cryoem at NYSBC CemGeneralInfo ...
Staff of the Cryoelectron Microscopy Facility Scientific Co Director: Bridget Carragher emg@nysbc.org tel: (212) 939 0660 ext 97241 fax: (212) 939 0863 webpage ...
How do I start? Note: The resources of the NYSBC are available for your use at no cost if you belong to a member institution. 1) Read thoroughly both the EM Introduction ...
How do I start? Note: The resources of the NYSBC are available for your use at no cost if you belong to a member institution. 1) Read thoroughly both the EM Introduction ...
Contents New Webpage Brief instructions for updating web pages manually ssh to cem100 Copy and paste the following commands to copy all pages: (~15 minutes ...
Contents Archive This is an archive of the website as of 2013.08.27 This is meant for people who need to use the public face of the website, but cannot access ...
Contents Listing of twiki pages to be automatically copied to nysbc web pages Instructions List any pages here that you want to be mirrored on our public site ...
twikipage TemimpsJobs InstallwxPython PlungeFreezing CemProtocPlungefreezing PPPioloformGrids PPFiducialsTomography PPCarbonSandwich ...
Listing of Main cryo EM webpage Instructions The main webpage without twiki names are made with specific templates, this is done with a script on cem100, /data ...
twikipage template CemEventsMainWeb index CemAboutMainWeb cem about CemStaff cem staff CemFormerStaff cem formerstaff CemSignupOverview ...
Help with the NYSBC Intranet (twiki) At the NYSBC we use an intranet to centralize, organize and store information. All users are required to create an account and ...
CEMUserhandbook ver1.pdf: CEM User Handbook version 1.00 EM User Handbook Note: Policies may have been updated since this handbook has been completed so please contact ...
Welcome to the CEM home page SAFETY at NYSBC CEM Newsletter EM Related Seminars and Courses New EMG sites Leginon / Appion EMG site New scheduler ...
Overview of the NYSBC Core Facilities NMR Facility X ray Facility Protein production Facility NYCOMPS Electron crystallography Center The Transcontinental EM Initiative ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Cryo Electron Microscopy Information and Instructions General Proper Handling of Cold Stages ...
Optical Bench and Scanner Instructions Darkroom Protocols Developing Film WEAR GLOVES!!! Protocol 1 Turn on red safe light by flipping switch down. 2 Fill ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Customise this topic; samples and ideas available at TWiki:TWiki.WebLeftBarCookbook. My links: My home page CEM facility page CEM Microscope Schedule ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Contents F20 Daily Checklist Cool microscope cold finger Insert holder to first stop Do not insert holder Open colum valves check for ...
Glow Discharge in Edwards 306 Recommended that Glow Discharge be carried out by the dedicated units You will need to install the plasma ring for this procedure ...
FEI Helios 650 The Helios is a state of the art SEM, equipped with a field emission gun and a focused ion beam, combining the very fine milling capabilities used in ...
Contents Ice Contamination Rate Test Theory All intensity measurements are proportional to number of electrons passing through sample. Measure them either directly ...
JEOL 1230 The JEOL 1230 is mainly used as a screening microscope. It has a tungsten filament and a side entry Erlangshen Gatan camera mounted at the 35mm port. The ...
JEOL 2100 The JEOL 2100F operates with an accelerating voltage of 200kV. It is equipped with a Schottky field emission gun and an array of three condenser lenses, ...
Contents JEOL 2100 Microscope Parameters wavelengths 200kV: 0.02508 A objective lenses f 2.8 mm Cs 2.0 mm Chromatic aberration 2.1 mm ...
JEOL 3200FSC The JEOL 3200FSC is a state of the art instrument that operates with an accelerating voltage of 300kV. It is equipped with a Schottky field emission gun ...
Contents JEOL 3200 training 3200 training notes.doc: DLS notes on 3200 training Cryogen fill Chuck dewar Fill in morning and last thing at night ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet CemEngProjects Personal Preferences ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Contents 22 January 2014 NYSBC webpage Joe revision mockup 01 NYSBC Website Index.jpg: 02 NYSBC Website Page.jpg: 15 January 2014 NYSBC webpage ...
EM Newsletter Archive Current edition: Spring 2014 2014 Spring 2014 2013 Winter 2013 Fall 2013 2012 Fall Winter 2012 Summer 2012 ...
Contents How to deal with uneven grid? 1) take a Z #8208;stack between a defined Z range 2) auto focus each image or every so often 3) use a focus surface 4 ...
Contents Nikon Ti U Inverted Light Microscope (basic instructions) Note: If there is a user after you, leave the Hg lamp on. Every time you turn on the Hg bulb ...
CEM Microscope Schedule CEM Microscope Allocations This process is summarized in CemSchedulingOverview Calendar below represents preallocation of microscope ...
CEM Microscope Schedule CEM Microscope Allocations 3 days of the week is allocated as summarized in CemSchedulingOverview 2 days of the week is allocated ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols TRIAFOL METHOD Jump to Principles Holey carbon grids ...
Contents Past EM Journal Clubs and seminar speakers 31 Oct 2011 4 pm Richard Henderson : MRC Laboratory of Molecular Biology, Cambridge, UK, "What is needed ...
Contents Responders to Emergency and Alert Calls full detail see EmergencyCall Set GROUP MichaelGoger, BorisItin, DhireshVyas, DanMurphy, RenatoBruni ...
List of NYSBC Intranet users Please take the time and add yourself to the list. To do that fill out the form in Registration. This will create an account for you ...
Tecnai F20 The Tecnai F20 operates with an accelerating voltage of 200kV. It is equipped with a Schottky field emission gun. The tip on this instrument was replaced ...
FEI TF20 Microscope Parameters wavelengths 200kV: 0.02508 A objective lenses f Cs 2.1 mm Chromatic aberration Aperture Sizes Condensor ...
Current Job Openings at NYSBC Entry level Technician Position in the Cryo Electron Microscopy Facility at the New York Structural Biology Center The Electron Microscopy ...
Contents Listing of twiki pages to be automatically copied to nysbc web pages Instructions List any pages here that you want to be mirrored on our public site ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Edwards 306 Vacuum Evaporator Jump to Principles Carbon ...
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