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2dx Overview Contents Protocols Computing... crash total computer: ssh from another machine sudo init 3 sudo kill 9 whatever Xorg sudo init ...
NYSBC CEMfac all hours access Contents How to acquire authorization for independent use of microscopes at any hour of the day The electron microscopes are in principle ...
Cryoelectron Microscopy of Macromolecular Assemblies 2006 NYU course # G16.4408 3 credits Contents Instructors David Stokes: stokes@saturn.med.nyu.edu ...
Cryoelectron Microscopy of Macromolecular Assemblies 2007 NYU course # G16.4408 3 credits for lectures, 1 credit for practicals credit also available at other ...
Cryoelectron Microscopy of Macromolecular Assemblies 2008 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...
Cryoelectron Microscopy of Macromolecular Assemblies 2009 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...
Cryoelectron Microscopy of Macromolecular Assemblies 2010 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...
Contents Instructors DavidStokes: stokes@saturn.med.nyu.edu BillRice: rice@nysbc.org IbanUbarretxena: iu@sci.ccny.cuny.edu HernandoJoseSosa: hsosa ...
Contents Fib/SEM CemFibSEMProcessing tif2mrc Image .tif rawstack.mrc xfalign matt 0.1 reduce 1 bilinear params 2 sobel rawstack.mrc rawstack.xf xftoxg n 0 rawstack ...
Contents Projects NYU Ned Seeman June 29, 2012 Initial meeting Steve Hubbard/Todd Miller February 23, 2012 First email from Steve March 9, 2012 ...
Contents CemMicroscopeTrainingArchive CEM microscope training sessions Open Training Days are microscope training sessions required before you may sign up for ...
Contents CEM microscope training sessions When there is a large enough group of new users CEM staff will hold a training/re training session. This training session ...
Contents User Guide for Robot/Leginon on JEOL 1230 Minghui Hu Friday, February 26, 2010 Minghui Leginon setup guide.doc: MS Word version PC access abc123 Checklists ...
Contents Ongoing issues Tecnai New apertures installed March 6 2015 Objective: 100 um, 50 um, 50 um, 40 um condenser: 150 um, 100 um, 70 um, 50 um BUT third ...
Single Particle Projects Random Conical Tilt (RCT) procedure methods used Oct 2012 Collect Data Prepare grids typically negative stain Use SerialEM to ...
Overview Interested in Cryoem? (How to begin a project) CemInterested Cryoem at NYSBC CemIntroduction General info on cryoem at NYSBC CemGeneralInfo ...
CEMUserhandbook ver1.pdf: CEM User Handbook version 1.00 EM User Handbook Note: Policies may have been updated since this handbook has been completed so please contact ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Cryo Electron Microscopy Information and Instructions General Proper Handling of Cold Stages ...
Contents F20 Daily Checklist Cool microscope cold finger Insert holder to first stop Do not insert holder Open colum valves check for ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tecnai Tomography Contents This Software Is NO LONGER IN USE at NYSBC: Use SerialEM Microscope ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Taking a film image Insert small screen adjust brightness until the exposure meter reads ...
GethinOwen 11 May 2006 Freeze sub solution method He.sxw: freeze substituion cryo low dose em.sxw: low dose em step by step holey carbon grids.sxw: Triafoil ...
Contents Ice Contamination Rate Test Theory All intensity measurements are proportional to number of electrons passing through sample. Measure them either directly ...
Return to Cryo EM Website at http://www.nysbc.org/facilities/CEM Cryo sample loading Contents Apertures Condenser aperture #2 Objective aperture #4 Check ...
Contents JEOL 2100 Controls, Comparison with Tecnai Hardware Controls JEOL Tecnai BEAM Gun Valve Open (software switch on Tecnai) OL ...
Contents JEOL 1230 Instructions Log in User Name: su Password: 1230 Specimen Insertion Flip toggle switch to "pump" (G) Line up pin on holder ...
JEOL 2100 Instructions Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM K2 Camera Regular Maintenance Anneal cycle should be run weekly ...
Contents New User Setup Tecnai F20 Create new accounts on both computers and Greymatter Set passwords to never expire Windows 2000 or XP ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM SerialEM Use Serial EM provided by David Mastronarde and colleagues from Univ. of Colorado, ...
Contents SerialEM Macros Following macros can be added by cut and paste into !SerialEM Macro Editor Auto Collection of many tomograms Need to have mid ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Contents Notes on Serial EM Use and Configuration on Tecnai F20 Steps to recording a Low Dose ...
Contents Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM To make a montage of your grid 1. Set magnification to 170X, maximum beam spread. ...
How to use Shutter box Control Application from TVIPS Background Tietz shutterbox has separate beam blank and camera shutter controls This allows beam to ...
Contents Sample Insertion InsertRoomTemperatureSample InsertCryoSample Tecnai F20 Problems Instructions to Retrieve CCDBlankEnable Film Jam Access ...
FEI TF20 Microscope Parameters wavelengths 200kV: 0.02508 A objective lenses f Cs 2.1 mm Chromatic aberration Aperture Sizes Condensor ...
Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tecnai F20 (basic operations) TEM data computer controls Fast Scan CCD holds data and provides ...
Contents TEMIMPS Workshop Schedule 2011 Monday Aug. 15 Tuesday Aug. 16 Wednesday Aug. 17 Thursday Aug 18 Friday Aug. 19 0830 Lecture: WelcomeLogistics and Introduction ...
Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tomography using TVIPS EM Menu 3.0 Contents This Software Is No Longer In Use at NYSBC : Use SerialEM ...
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