Cryo electron Microscopy (CEM) uses electrons accelerated to almost the speed of light to reveal images of individual molecules and molecular assemblies. The electrons ...

Cryoelectron Microscopy (CEM) uses electrons accelerated to almost the speed of light to reveal images of individual molecules and molecular assemblies. The electrons ...

Procedure for using the CEM facility at the NYSBC The NYSBC The New York Structural Biology Center is a 501 (c)(3) corporation incorporated in the State of New York ...

Contents Ed's Overview of EM (cartoon version) Choice of an appropriate structural biological technique Before beginning a project one must match the biological ...

Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 10th year at NYSBC Fall ...

Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered at NYSBC Fall 2005 Description: A comprehensive course in the theory and practice of ...

Visit the !CryoEM web site at http://www.nysbc.org/facilities/CEM Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 4th year at ...

Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 4th year at NYSBC Fall ...

Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 5th year at NYSBC Fall ...

Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 6th year at NYSBC Fall ...

Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 7th year at NYSBC Fall ...

Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 8th year at NYSBC Fall ...

Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 9th year at NYSBC Fall ...

Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 10th year at NYSBC Fall ...

CEM Course Group Web Site for Graduate Course "CryoElectron Microscopy of Macromolecular Complexes" CemCourseAnnouncement CemCourseAnnouncement14 CemCourseSyllabus14 ...

Visit Cryo EM website at http://www.nysbc.org/facilities/CEM Course Materials for Graduate Course "CryoElectron Microscopy of Macromolecular Complexes" Dec 6 ...

Visit Cryo EM website at http://www.nysbc.org/facilities/CEM Course Materials for Graduate Course "CryoElectron Microscopy of Macromolecular Complexes" Dec. 5 ...

Visit Cryo EM website at http://www.nysbc.org/facilities/CEM Course Materials for Cryoelectron Microscopy of Macromolecular Assemblies COURSE EVALUATION CEM ...

Contents Dec 9 Visualization, Interpretation and Docking (Hernando Sosa) Lecture Presentation lecture dec9.mp3: audio of Dec 9 lecture Dec 2 Digital ...

Contents Class Evaluation form CEM Course evaluation 2009.doc: Course Evaluation Dec 8 Fitting Hernando Sosa Lecture Presentation Fitting EM12 ...

Contents Dec 7 Molecular Fitting Lecture Presentation sosa2.mp3: audio of lecture on molecular fitting Nov 23 Helical Reconstruction Lecture ...

Contents Dec. 7 Molecular Fitting Willy Wriggers Lecture NYSBC11.ppt: Molecular Fitting PPT Molecular fitting 20111206 2105 1.arf: recording ...

Contents Dec 4 Molecular Fitting Willy Wriggers NYSBC12.ppt: Molecular fitting lecture Wriggers 2012 Molecular Fitting 20121204 2102 1.arf: Molecular ...

Contents Dec 10 Molecular Fitting Wily Wriggers Lecture nysbc13.ppt: powerpoint of molecular fitting lecture wriggers.mp3: audio of molecular ...

Contents Player required for arf video files nbr2player.msi: MS windows player for arf files Lectures Structure Validation and Fitting Dec. 9 Lecture ...

Overview Detailed Syllabus: Jan 11 : Introduction SEMC tour Welcome to the Simons Electron Microscopy Center and explanation of the course. A tour of NYSBC and ...

CEM Course Paper Topics Sign up for Paper Topics in the table below: Go to the bottom of this page. Hit the "Edit" link. Enter your name in between ...

CEM Course Paper Topics Final papers are due on Dec 13. Send pdf to stokes@nyu.edu Papers should be no more than 5 pages of text for the body. Figures ...

CEM Course Paper Topics Final papers are due on Dec 11. Send pdf to stokes@nyu.edu Papers should be no more than 5 pages of text for the body. Figures ...

CEM Course Paper Topics Final papers are due on Dec 12. Send pdf to stokes@nyu.edu Papers should be no more than 5 pages of text for the body. Figures ...

CEM Course Paper Topics Final papers are due on Dec 11. Send pdf to stokes@nyu.edu Papers should be about 5 pages of text for the body. Figures should ...

Final papers are due on Dec 13. Send pdf to stokes@nyu.edu Papers should be about 5 pages of text for the body. Figures should be included to illustrate ...

Final papers are due on Dec 15. Send pdf to stokes@nyu.edu Papers should be about 5 pages of text for the body. Figures should be included to illustrate ...

Final papers are due on Dec 20. Send pdf to stokes@nyu.edu Your paper should be about 5 pages of text for the body. Figures should be included to illustrate ...

Contents Final papers are due on Dec 20. Send pdf to stokes@nyu.edu Your paper should be about 5 pages of text for the body. Figures should be included ...

Contents Student list Name Email Position School credit (yes/no) Thomas Hsiao thsiao@mail.rockefeller.edu Grad Student Rockefeller ...

Cryoelectron Microscopy of Macromolecular Assemblies 2005 NYU course G16.2617001 Call #30137 3 credits Contents Instructors David Stokes: stokes@saturn ...

Cryoelectron Microscopy of Macromolecular Assemblies 2006 NYU course # G16.4408 3 credits Contents Instructors David Stokes: stokes@saturn.med.nyu.edu ...

Cryoelectron Microscopy of Macromolecular Assemblies 2007 NYU course # G16.4408 3 credits for lectures, 1 credit for practicals credit also available at other ...

Cryoelectron Microscopy of Macromolecular Assemblies 2008 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...

Cryoelectron Microscopy of Macromolecular Assemblies 2009 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...

Cryoelectron Microscopy of Macromolecular Assemblies 2010 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...

Contents Practical Sessions Homework : preparation for practical session II Download and install IMOD on your mac/pc/linux system Get the latest stable ...

Contents Instructors DavidStokes: stokes@saturn.med.nyu.edu BillRice: rice@nysbc.org IbanUbarretxena: iu@sci.ccny.cuny.edu HernandoJoseSosa: hsosa ...

Contents Syllabus Intro to EM Optics, image formation and Diffraction Sept 23 David Stokes (NYU/NYSBC) Fourier transforms, CTF, cross correlation Sept 30 ...

Contents List of Samples Carbon Film Gold TMV : single particle helical reconstruction phi6 (Paul Gottlieb) : single particle, icosahedral reconstruction ...

Signup for Discussion sessions of Cryoelectron Microscopy Class Please edit this page and check Discussion Session that you would like to present Then hit save ...

Signup for Discussion sessions of Cryoelectron Microscopy Class Please edit this page and enter your TWiki username next to the Discussion Session that you would ...

Signup for Discussion sessions of Cryoelectron Microscopy Class Please edit this page and enter your TWiki username next to the Discussion Session that you would ...

Investigators interested in using the Dual Beam SEM Dave Hall/Scott Emmons hall@aecom.yu.edu, emmons@aecom.yu.edu (718 430 3130) Hall: biosketch, othersupport ...

Electron Tomography Projects Influenza: indentifying structural characteristics in egg adapted vaccine preparations A 7Å thick section through a tomographic ...

Capabilities of NYSBC's CEM Lab Printable Brochure General Information The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate ...

Capabilities of NYSBC's CEM Lab General Information The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate the intermolecular interactions ...

Contents Projects on the Nanolab Characterization of organelles in identified cells in the nematode, C. elegans PI: Dave Hall Institution: Albert Einstein ...

Contents Homework Uploads To get the most out of this course, it is required to do all of the practical sessions Students taking the course for credit are ...

IT resources at the CEMfac List of supported SoftWare EmIP GUI for image processing Tomography MontageNavigator hints on using the Navigator in SerialEM ...

Beginning an electron microscopy project High Purity? Usually, a project aimed to determine structural information of a given material is ripe for study when a high ...

Beginning an electron microscopy project High Purity? Usually, a project aimed to determine structural information of a given material is ripe for study when a high ...

Capabilities of NYSBC's CEM Lab Printable Brochure Introduction The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate the intermolecular ...

Visit Cryo EM website at http://www.nysbc.org/facilities/CEM !CryoEM facility at NY Structural Biology Center CapabilitiesCEM.doc: MS Word version CapabilitiesCEM ...

Contents CEM Friday Evening Lecture Series 2008 CEM Friday Evening lecture series: Date Speaker INST; Title June 6 – Martin Vink NYSBC; High Throoughput crystallyzation ...

Dear Committee members, Further to my last message, I am attaching the documents recently sent to our two potential vendors requesting their Best and Final Offers ...

CEM Microscope Schedule Please Use the New Scheduler If you do not have an account, email emg@nysbc.org Temporary EM Microscope Requests http://emg.nysbc ...

Capabilities of NYSBC's CEM Lab General Information The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate the intermolecular interactions ...

Contents CEM Practical Signup and Information place and time Practicals will be from 10 am to 1 pm, Oct 29 Nov. 2 (single particle reconstruction and tomography ...

Contents Signup list: Please add your name to the list Practical session I from Nov. 4 8, 11AM 2PM Topics include programs to process data for: ...

Name Type Size Values Tooltip message Attributes Title of Project text 60 enter a title PI of Lab text 40 specify ...

Name Type Size Values Tooltip message Attributes PART I label A. Title of Project text 80 enter a title M B. PI ...

Principles and Protocols for EM structural studies Electron microscopy techniques Structural biology using Transmission Electron Microscopy (TEM) Electron ...

Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles EM Tomography Sample Prep Considerations: because life ...

Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Grid Preparation Solid Carbon Films Triafol Holey ...

Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Electron Tomography Jump to Principles Imod / Etomo Protomo ...

Principles Protocols Single Particle For single particle projects, we are using two packages: SPIDER and EMAN In general, EMAN is easier to use and get ...

Principles and Protocols for EM structural studies Electron microscopy techniques Structural biology using Transmission Electron Microscopy (TEM) Electron ...

Published work using the NYSBC Cryo EM Facility. Examples of work done at the NYSBC List of Publications Examples of work done at the NYSBC     ...

Current NYSBC Research Projects Collaborations Electron crystallography Helical reconstruction Single particle analysis ...

Courses and Seminars related to !CryoEM At the NYSBC CEM User meeting and Annual BBQ Event: CEM user meeting Location: NYSBC, Room A 11 Time: Friday, August 22, ...

(Archive)Courses and Seminars related to !CryoEM 2013 At the NYSBC Semester long Course : "Cryoelectron Microscopy of Macromolecular Assemblies" This comprehensive ...

Procedure for using the CEM facility at the NYSBC The NYSBC The New York Structural Biology Center is a 501 (c)(3) corporation incorporated in the State of New York ...

Procedure for using the CEM facility at the NYSBC The NYSBC The New York Structural Biology Center is a 501 (c)(3) corporation incorporated in the State of New York ...

Overview Interested in Cryoem? (How to begin a project) CemInterested Cryoem at NYSBC CemIntroduction General info on cryoem at NYSBC CemGeneralInfo ...

Contents Listing of twiki pages to be automatically copied to nysbc web pages Instructions List any pages here that you want to be mirrored on our public site ...

twikipage TemimpsJobs InstallwxPython PlungeFreezing CemProtocPlungefreezing PPPioloformGrids PPFiducialsTomography PPCarbonSandwich ...

CEMUserhandbook ver1.pdf: CEM User Handbook version 1.00 EM User Handbook Note: Policies may have been updated since this handbook has been completed so please contact ...

Cryosectioning notes from Chyongere Hirsch (Wadsworth Center Albany,NY)24 Mar 05 Grids 200 mesh Quantifoil for tomography Glow discharge Carbon coat Gold ...

Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Cryo Electron Microscopy Information and Instructions General Proper Handling of Cold Stages ...

Equipment for Sample Preparation Sample Preparation for Cryo EM Equipment BOC Edwards Vacuum Evaporator HighPressFreezer PlungeFreezing Freeze ...

Name: David Stokes Email: stokes@nyu.edu Company Name: NYU Company URL: http://stokeslab.med.nyu.edu Location: Skirball Institute 3 13 Country ...

Statement: A dual beam scanning electron microscope is requested for creating large scale reconstructions of tissue. This instrument will be situated at the New York ...

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Dual Tilt axis tomography on the JEOL 3200FSC New How to use the dual tilt holders of the ...

EM Imaging Processing GUI EMIP EMIP is a Graphical User Interface written in wxPython that collects information from the user and runs existing programs from ...

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tecnai Tomography Contents This Software Is NO LONGER IN USE at NYSBC: Use SerialEM Microscope ...

Contents Assessment of FIB instruments Visit to NIH Overall The instrument at the NIH is a FEI Quanta SEM with a focused ion beam. The focused ion beam is the usual ...

GethinOwen 11 May 2006 Freeze sub solution method He.sxw: freeze substituion cryo low dose em.sxw: low dose em step by step holey carbon grids.sxw: Triafoil ...

Name: Guobin Hu Email: ghu@saturn.med.nyu.edu Company Name: NYUSOM Company URL: http://www. Location: NYUSoMOffice Country: USA Comment ...

Contents Kdp plot defocus values for individual images from each dataset (df vs. tilt angle) motif averaging of David's and Guobin's data (maybe later ...

FEI Helios 650 The Helios is a state of the art SEM, equipped with a field emission gun and a focused ion beam, combining the very fine milling capabilities used in ...

META GROUP MEETINGS – NYSBC Held in A 11 Park Building at 1:30 pm unless noted otherwise New in 2006 Round Table discussions of technical topics and application ...

Mar 29 NOON (a) Goldbourt (McDermott Group) "Magic Angle Spinning Solid State NMR Studies of Intact Virus: Assignment of the Coat Protein of the 35MD Pf1 Filamentous ...

Contents Kent Notes indicators of good freeze smooth membranes round organelles indicators of bad freeze Echlin Book has good ...

Laboratory of Physical Biochemistry Other 2011 Ndao, M., Dutta, K., Bromley, K. M., Lakshminarayanan, R., Sun, Z., Rewari, G., Moradian Oldak, J., and Evans ...

2011 Ndao, M., Dutta, K., Bromley, K. M., Lakshminarayanan, R., Sun, Z., Rewari, G., Moradian Oldak, J., and Evans, J. S. (2011) Probing the self association, intermolecular ...

Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Fiducial Markers for Tomography Jump to Principles Gold ...

Contents Past EM Journal Clubs and seminar speakers 31 Oct 2011 4 pm Richard Henderson : MRC Laboratory of Molecular Biology, Cambridge, UK, "What is needed ...

Customise this topic; samples and ideas available at TWiki:TWiki.WebLeftBarCookbook. My links: My home page microscope schedule Microscope request ...

2Dcrystallization.doc: SERCA/PLB 2D Crystallization Protocol 2Dcrystallography.doc: 2D Electron Crystallography Processing Procedure TomographyProcessing ...

Name Type Size Values Tooltip message Attributes Title text 60 enter a title Investigator text 40 specify P.I. ...

For copyright reasons, you will not be able to access articles which are not on PubMedCentral on computers outside the networks of the Member Institutions NYSBC ...

For copyright reasons, you will not be able to access articles which are not on PubMedCentral on computers outside the networks of the Member Institutions NYSBC ...

For copyright reasons, you will not be able to access articles which are not on PubMedCentral on computers outside the networks of the Member Institutions NYSBC ...

For copyright reasons, you will not be able to access articles which are not on PubMedCentral on computers outside the networks of the Member Institutions NYSBC ...

Nov 2008 Scientific Report, NYSBC Figure contents described on last page. Introduction In order to provide Principal Investigators and ...

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM SerialEM Use Serial EM provided by David Mastronarde and colleagues from Univ. of Colorado, ...

Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Contents Notes on Serial EM Use and Configuration on Tecnai F20 Steps to recording a Low Dose ...

PROTOCOLS from Stokes Lab Members Cell Culture yeast growth protocol vink.doc: Yeast Culture (Martin Vink) 12/2007 Yeast cultures.doc: Yeast culture protocol ...

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tecnai F20 (basic operations) TEM data computer controls Fast Scan CCD holds data and provides ...

Current Job Openings at NYSBC Entry level Technician Position in the Cryo Electron Microscopy Facility at the New York Structural Biology Center The Electron Microscopy ...

Workshop in Dual Beam Scanning Electron Microscopy at the New York Structural Biology Center This workshop will introduce the new Helios 650 Nanolab microscope at ...

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tomography using TVIPS EM Menu 3.0 Contents This Software Is No Longer In Use at NYSBC : Use SerialEM ...

2dx UMA 2D crystallization Properties Amino acid sequence: MKLRLTDLAGPVGMGIFMLVVQILSLLLVAPLTDYYDLKAFENPQSVWNPVFYIVLIILFSAVLLLIIKFNMKWIIQLFMIFAVFSTLIYVLFGMGTMLLPPPQYGWEIILAASIIISILLTALMVVYPEWYIVDAVGIMVAAGASALFGISLSVIPTIILLVLLAVYDFIAVYKTKHMIKLAEGVMDLKLPILFVLPRKLSYSYVRSRTQKLEPGKEREAYFMGLGDAVMPTVLAVSASAFLEAPKVLGFANVPAIGVILGTLVSYAVLMYFVMKGKPQAGLPFLCTGAIAGFLLGCLAAGINPFF ...