My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Links My Logbook .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
How to do things at NYSBC \ Facilities and Laboratories at NYSBC ("Groups") Notices to Affiliates and Staff Get Affiliate Statistics (slow) Programs ...
(15.4; 688.1; 0.1) Robot Manuals (leave this at the top) Controller manual RCX142 e.pdf: rcx142 manual (this is most useful) RCX143 144 e 0322 v1.02.pdf ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
Contents TEMIMPS Workshop Schedule 2011 Monday Aug. 15 Tuesday Aug. 16 Wednesday Aug. 17 Thursday Aug 18 Friday Aug. 19 0830 Lecture: WelcomeLogistics and Introduction ...
Contents Listing of twiki pages to be automatically copied to nysbc web pages Instructions List any pages here that you want to be mirrored on our public site ...
Contents blank minutes page.doc: template for minutes TEMIMPS PImtg Oct 10 2012.txt TEMIMPS PImtg Sep 4 2012.txt PI Meeting in Washington DC 7/2012 TEMIMPS ...
Google Analytics Log In: user name temimps@gmail.com password temimps1 Mail from temimps account currently forwarded to: kderr@nysbc.org Set ALLOWTOPICVIEW ...
List of NYSBC Intranet users Please take the time and add yourself to the list. To do that fill out the form in Registration. This will create an account for you ...
How to use Shutter box Control Application from TVIPS Background Tietz shutterbox has separate beam blank and camera shutter controls This allows beam to ...
My Links My Logbook .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
Contents Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM To make a montage of your grid 1. Set magnification to 170X, maximum beam spread. ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Contents Notes on Serial EM Use and Configuration on Tecnai F20 Steps to recording a Low Dose ...
My Links My Logbook .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
Contents Comparison between Albany and NYSBC HPM 010 andreasample1.png: Figure 1: Conventionally, chemically fixed corpus callosum versus high pressure frozen ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Using the PDS 1010GM Microdensitometer Download MSWord file ...
Contents Past EM Journal Clubs and seminar speakers 31 Oct 2011 4 pm Richard Henderson : MRC Laboratory of Molecular Biology, Cambridge, UK, "What is needed ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Solid Carbon Film Jump to Principles Using Mica (Best ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Positive Staining Jump to Principles You will need: ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Prepare Pioloform Grids Jump to Principles 1. Clean a ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Negative Staining Jump to Principles You will need: ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Fiducial Markers for Tomography Jump to Principles Gold ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Formvar Holey Film Jump to Principles This method has a ...
Contents Optical Bench DO NOT LOOK DIRECTLY INTO BEAM!!! Normal Use Turn on power strip Put in standard and record image Put in negative of interest ...
Various NYSBC Procedures New Affiliate Endorsement Procedures for setting up Teleconference and remote PC viewing Procedures for using Preapproved PO's ...
Contents Posters for use at meetings for NYSBC There probably should be specific ones for all areas, and general ones also..., but we start with NmrPoster and General ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Microtome Conversion Remove Cryosectioning unit Bake ...
Contents Microscope Tests New performance testing page Measuring Ice Contamination Rates Drift Test Assessment of FIB instruments Set ALLOWTOPICVIEW ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
Matlab Oscilloscope Parameters Wait until computer connects to network Open Matlab 7.0.1 Open HPF.si file Horizontal scale 500ms/div or 100ms/div Horizontal Scale ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
Contents Maintenance Instructions for 14 day tasks Week A Vacuum F20 and 1230 rooms Regrease O rings and clean All Microscope Holders Only Use Fomblin Grease ...
Contents Maintenance Instructions for 30 day items Week I Drain Air Compressors (4) 2100, 1230, 3200 and robot Week II Bake Cryo Holders for 12 hours Week ...
Contents Maintenance Instructions for 18 Month Tasks Adixen Molecular Drag Pump model MDP 5011 for Gatan Dry Pumping Station Operating and maintenance tips to maximize ...
Contents Maintenance Instructions for 12 Month Tasks Purification of Direct Q3 water system Items you will need before beginning: Smartpak filter (back ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Logbook Patrick Barre's Project proposal My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Name: KD Derr Email: kderr@nysbc.org Company Name: NYSBC Company URL: http://www.nysbc.org Location: ParkBuildingOffice Serial EM Support Resources ...
Contents User Instructions for 3200FSC These are for users who come to use the microscope Assumed that the microscope has already been aligned and software ...
JEOL 2100 Instructions Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM K2 Camera Regular Maintenance Anneal cycle should be run weekly ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on the style of Intranet (TWiki ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols High Pressure Freezing Jump to Principles Equipment Instructions ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
Glow Discharge in Edwards 306 Recommended that Glow Discharge be carried out by the dedicated units You will need to install the plasma ring for this procedure ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Freeze Substitution Jump to Protocol Important considerations ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Freeze Substitution Jump to Principles Instructions for ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
Contents Instructions for finding the beam Move sample to make sure you are not on a grid bar If you are using the cryostage make sure the shutter is ...
Screening and Scanning EM Film Instructions for PDS 1010GM Microdensitometer Instructions for PDS 1010GM Data Processing Instructions for Zeiss Photoscan ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Taking a film image Insert small screen adjust brightness until the exposure meter reads ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
Name: Elena Yakubovskaya Email: elena@pharm.stonybrook.edu Company Name: SUNY Stony Brook Company URL: http://www.pharm.stonybrook.edu/Faculty/Professors ...
CUNY excavation. Change of schedules, All hours, late night, weekend, and holiday access. EM users should be aware of possible Impact until further notice. The detailed ...
CryoUltraMicrotomy Microtome Conversion Cryosectioning notes from Chyongere Hirsch (Wadsworth Center Albany,NY)24 Mar 05 Visit the Cryo EM Web site at ...
Contents March 2010 We will be given 1 month notice before final trench is dug. Anticipated date is not included in current construction schedules. Cryo EM As ...
Contents CEM construction contingency plan General information CUNY will build a new laboratory building adjacent to the NYSBC. Although the plan was for the construction ...
Contents Cold Stages You must be trained on proper handling of the cold stages before unattended use is permitted. Gatan This holder easily can obtain 1nm ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
Courses and Seminars related to !CryoEM At the NYSBC CEM User meeting and Annual BBQ Event: CEM user meeting Location: NYSBC, Room A 11 Time: Friday, August 22, ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Background of structural biology The objective of structural ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Negative Staining Jump to Protocol Why Negative stain ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Single Particle Analysis Jump to Protocol Three dimensional ...
Principles Protocols Single Particle For single particle projects, we are using two packages: SPIDER and EMAN In general, EMAN is easier to use and get ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Single Particle Analysis Jump to Principles Spider EMAN ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Electron Tomography Jump to Principles Imod / Etomo Protomo ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Electron Crystallography Jump to Principles 2dx subsub ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Helical Reconstruction Jump to Protocol Set ALLOWTOPICCHANGE ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles High Pressure Freezing Jump to Protocol Biological specimens ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles EM Tomography Sample Prep Considerations: because life ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Electron crystallography Jump to Protocol In order to ...
Proposal form for a new CEM project Directions Read CEM Introduction and Interested in EM? before filling out this form Click on the gray box: "Create ...
Contents Signup list for practicals Please add your name to the table so we can get an idea of how many will be attending the practicals (week of Nov. 3). Table ...
Rules for Requests 1 Priority for each microscope is given according to preallocated schedule CemMicroscopeSchedule confirmed bookings are also shown on ...
Capabilities of NYSBC's CEM Lab Printable Brochure Introduction The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate the intermolecular ...
IT resources This page is now broken into two parts: CemITSystem for system admin related issues CemITApplications for issues related to EM software Set ALLOWTOPICVIEW ...
Request Page for CUNY EM Users This page is for requesting pre allocated EM time slots, which are indicated on CemMicroscopeSchedule you may read rules for how ...
Contents IF CCD blank enable window is closed, then CCD blanking box won't work Occasionally, box won't work even though CCD blank Enable is open and checked ...
Catalase Crystals Recrystallization Protocol You will need Catalase from Bovine liver Vendor: Sigma CAS Number: 9001 05 2 Dialysis buttons or slides ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Using Carbon Rod in Edwards 306 Jump to Principles THIS ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Carbon Coating Jump to Protocol The best method to use ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
MyLogbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
My Links My Logbook .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
NYSBC CEMfac all hours access Contents How to acquire authorization for independent use of microscopes at any hour of the day The electron microscopes are in principle ...
My Links My Logbook .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
Contents Making Affinity Grids Supplies Teflon Block with flat bottom ala Diane Taylor Ni NTA continuous carbon (neg stain) or Quantifoil (cryo) grids ...
NYSBC Affiliation procedure for individual users How To Become Affiliated with NYSBC In order to become Affiliated with NYSBC, you need endorsement from ...
1230 Robot checklist Robot 1 turn on gas tank 1 start iRobot and hist "Start" 1 demagnitize grid pickup nozzle 1 clamp grid tray in position 1 place ...