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1230 Robot checklist Robot 1 turn on gas tank 1 start iRobot and hist "Start" 1 demagnitize grid pickup nozzle 1 clamp grid tray in position 1 place ...
2dx Overview Contents Protocols Computing... crash total computer: ssh from another machine sudo init 3 sudo kill 9 whatever Xorg sudo init ...
AFFILIATION ENDORSEMENT AT NYSBC Your notes Please change this text to indicate any additional information not reflected on the form below Endorsement ...
AFFILIATION ENDORSEMENT AT NYSBC Your notes Please change this text to indicate any additional information not reflected on the form below ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
List AllHoursList OPERATIONS OUTSIDE NORMAL WORKING HOURS All hours access. 1.Purpose and General Comments 1 Purpose. The resources of NYSBC need to be ...
NYSBC CEMfac all hours access Contents How to acquire authorization for independent use of microscopes at any hour of the day The electron microscopes are in principle ...
My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest starting ...
Contents Loading MRC files: Need to select RAW data need to tell it image size (from header) Use "float", data type 1 header is 1024 bytes Loading ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Name: Andrea Nans Email: andrea.nans@gmail.com Company Name: New York University Company URL: http://saturn.med.nyu.edu/research/sb/stokeslab/ ...
Name: Andrew Pomfret Email%: Company Name: NYU School of Medicine Company URL: http://saturn.med.nyu.edu/~stokes/ Location: NYUSoMOffice ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Carbon Coating Jump to Protocol The best method to use ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Using Carbon Rod in Edwards 306 Jump to Principles THIS ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Using Carbon Thread in Edwards 306 Jump to Principles ...
Contents IF CCD blank enable window is closed, then CCD blanking box won't work Occasionally, box won't work even though CCD blank Enable is open and checked ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Taking CCD Image with EMMENU Blank beam Click to expose in Tecnai interface ...
Contents 2013 CEM webpage refresh Background Refresh of CEM webpage to accommodate NYSBC new template. Architecture Public CemTwikiToWebList CemTwikiToWebMainPageTest ...
Procedure for using the CEM facility at the NYSBC The NYSBC The New York Structural Biology Center is a 501 (c)(3) corporation incorporated in the State of New York ...
Member institution core electron microscopy facilities Albert Einstein College of Medicine of Yeshiva University City University of New York Columbia ...
CEM Administration Contacts CEMStaffEmergencyContact EmergencyCall Staff Procedures Ordering, Shipping and Receiving NYSBC Procedures ...
Contents NEW DUAL BEAM FIB/SEM MICROSCOPE AVAILABLE FOR YOUR USE The New York Structural Biology Center (NYSBC), to which your institution belongs, has recently acquired ...
NEW DUAL BEAM FIB/SEM MICROSCOPE AVAILABLE FOR YOUR USE The New York Structural Biology Center (NYSBC), to which your institution belongs, has recently acquired ...
Contents Processing FIB SEM data Make a stack of images 1 Copy data over from microscope 1 Select useful images 1 Make an mrc stack from several tif images ...
Contents Ed's Overview of EM (cartoon version) Choice of an appropriate structural biological technique Before beginning a project one must match the biological ...
Contents Administration logs Jan 2011 Set up and installed new computer, cem15 CentOS 5.5, RAID5, 5.2 TB disk All standard EM software installed ...
Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 10th year at NYSBC Fall ...
Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered at NYSBC Fall 2005 Description: A comprehensive course in the theory and practice of ...
Download the flier CemCourseAnnounce.pdf: Cryo EM Course flier Set ALLOWTOPICVIEW DavidStokes 01 Aug 2006
Visit the !CryoEM web site at http://www.nysbc.org/facilities/CEM Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 4th year at ...
Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 4th year at NYSBC Fall ...
Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 5th year at NYSBC Fall ...
Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 6th year at NYSBC Fall ...
Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 7th year at NYSBC Fall ...
Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 8th year at NYSBC Fall ...
Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 9th year at NYSBC Fall ...
Visit the !CryoEM web site at http://cryoem.nysbc.org Cryoelectron Microscopy of Macromolecular Assemblies Graduate Course offered for the 10th year at NYSBC Fall ...
CEM Course Group Web Site for Graduate Course "CryoElectron Microscopy of Macromolecular Complexes" CemCourseAnnouncement CemCourseAnnouncement14 CemCourseSyllabus14 ...
Visit Cryo EM website at http://www.nysbc.org/facilities/CEM Course Materials for Graduate Course "CryoElectron Microscopy of Macromolecular Complexes" Dec 6 ...
Visit Cryo EM website at http://www.nysbc.org/facilities/CEM Course Materials for Graduate Course "CryoElectron Microscopy of Macromolecular Complexes" Dec. 5 ...
Visit Cryo EM website at http://www.nysbc.org/facilities/CEM Course Materials for Cryoelectron Microscopy of Macromolecular Assemblies COURSE EVALUATION CEM ...
Contents Dec 9 Visualization, Interpretation and Docking (Hernando Sosa) Lecture Presentation lecture dec9.mp3: audio of Dec 9 lecture Dec 2 Digital ...
Contents Class Evaluation form CEM Course evaluation 2009.doc: Course Evaluation Dec 8 Fitting Hernando Sosa Lecture Presentation Fitting EM12 ...
Contents Dec 7 Molecular Fitting Lecture Presentation sosa2.mp3: audio of lecture on molecular fitting Nov 23 Helical Reconstruction Lecture ...
Contents Dec. 7 Molecular Fitting Willy Wriggers Lecture NYSBC11.ppt: Molecular Fitting PPT Molecular fitting 20111206 2105 1.arf: recording ...
Contents Dec 4 Molecular Fitting Willy Wriggers NYSBC12.ppt: Molecular fitting lecture Wriggers 2012 Molecular Fitting 20121204 2102 1.arf: Molecular ...
Contents Dec 10 Molecular Fitting Wily Wriggers Lecture nysbc13.ppt: powerpoint of molecular fitting lecture wriggers.mp3: audio of molecular ...
Contents Player required for arf video files nbr2player.msi: MS windows player for arf files Lectures Structure Validation and Fitting Dec. 9 Lecture ...
Attendees name position inst lab email credit reg card disc Ana Asenjo tech AECOM Sosa Lab aasenjo@aecom.yu.edu ...
Attendees name position inst lab email credit disc paper Allyson Bunbury grad stud AECOM Pathology abunbury@aecom.yu ...
Cryoelectron Microscopy of Macromolecular Assemblies 2005 NYU course G16.2617001 Call #30137 3 credits Contents Instructors David Stokes: stokes@saturn ...
Cryoelectron Microscopy of Macromolecular Assemblies 2006 NYU course # G16.4408 3 credits Contents Instructors David Stokes: stokes@saturn.med.nyu.edu ...
Cryoelectron Microscopy of Macromolecular Assemblies 2007 NYU course # G16.4408 3 credits for lectures, 1 credit for practicals credit also available at other ...
Cryoelectron Microscopy of Macromolecular Assemblies 2008 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...
Cryoelectron Microscopy of Macromolecular Assemblies 2009 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...
Cryoelectron Microscopy of Macromolecular Assemblies 2010 NYU course # G16.4408 4 credits credit also available at other institutions Instructors DavidStokes ...
Contents List of Samples Carbon Film Gold TMV : single particle helical reconstruction phi6 (Paul Gottlieb) : single particle, icosahedral reconstruction ...
Contents Fib/SEM CemFibSEMProcessing tif2mrc Image .tif rawstack.mrc xfalign matt 0.1 reduce 1 bilinear params 2 sobel rawstack.mrc rawstack.xf xftoxg n 0 rawstack ...
Using cryo EM equipment at the NYSBC Equipment overview Sample Preparation Electron Microscopy Equipment signup Overview for Equipment Scheduling ...
Cryoelectron Microscopy of Macromolecular Assemblies 12/2/2014: Helical reconstruction Time: 4:00 6:00 pm Instructor: Hernando Sosa (Albert Einstein College of ...
Contents Extracting all files from a SerialEM stack run the script /cryoem/script/extract.pl mystack.st will automatically extract all files in stack ...
Contents Slice and View Images Images collected using slice and view are saved as a series of 8 bit tiffs Since contrast on SEM is inverted from usual TEM ...
CryoEM Grant Applications TwoDCrystalScreening NanoMedicine NIH Electron Grant Application Process CemDualBeam Visit Cryo EM website at http://www ...
Contents Fei Helios Instructions Sample prep Samples come in resin blocks Trim block size These generally need to be trimmed to ~1cm height so they will ...
Contents Projects on the Nanolab Characterization of organelles in identified cells in the nematode, C. elegans PI: Dave Hall Institution: Albert Einstein ...
Capabilities of NYSBC's CEM Lab Printable Brochure Introduction The goal of the NYSBC cryoelectron microscopy facility is to help researchers elucidate the intermolecular ...
Visit Cryo EM website at http://www.nysbc.org/facilities/CEM !CryoEM facility at NY Structural Biology Center CapabilitiesCEM.doc: MS Word version CapabilitiesCEM ...
Dear Committee members, Further to my last message, I am attaching the documents recently sent to our two potential vendors requesting their Best and Final Offers ...
Dear Committee members, Exciting things are happening at NYSBC. Really. About 10 trucks of concrete were here last week and left us with footings for the new building ...
Contents Networking nfs mounts made directories /cem02bigdata/, cem03bigdata/, /cem12data/, ... change group of each one from "staff" to "wheel" under ...
CryoEM Administration Cowburn's email list June 11,2004: cowburn science email.txt See also DGmailList NYU email lists (moderated) postdoc@lists ...
Contents CemMicroscopeTrainingArchive CEM microscope training sessions Open Training Days are microscope training sessions required before you may sign up for ...
Contents General Questions Question: I have a protein and want to do EM, what do I do? If you have a protein or a protein complex that has a MW ~300kDa ...
Contents Nikon Ti U Description Users have access to a Nikon Ti U inverted fluorescence microscope with a FEI Cryo Stage2 system. It allows screening of electron ...
Visit the !CryoEM web site at http://www.nysbc.org/facilities/CEM Cryo EM Operations Committee Members Your representative from the !CryoEM Operations Committee ...
Visit the !CryoEM web site at http://www.nysbc.org/facilities/CEM Cryo EM Operations Committee Members Your representative from the !CryoEM Operations Committee ...
Cryo Electron Microscopy Information and Instructions Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM Contents wavelengths 80kV: 0.04176 ...
USER YuZhao Your name will automatically appear as the creator of this new CryoEM Project. You will shortly receive an email copy of the confirmation of ...
Proposal form for a new CEM project Directions Read CEM Introduction and Interested in EM? before filling out this form Click on the gray box: "Create ...
USER Your name will automatically appear as the creator of this new CryoEM Project. Only you and the CEM staff have access to view the contents of this document ...
USER Your name will automatically appear as the creator of this new CryoEM Project. You will shortly receive an email copy of the confirmation of your project ...
Welcome to NYSBC We have received your proposal for using the electron microscope facility at NYSBC. If you have not already discussed this project with one of the ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Electron crystallography Jump to Protocol In order to ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles EM Tomography Sample Prep Considerations: because life ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Grid Preparation Solid Carbon Films Triafol Holey ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles High Pressure Freezing Jump to Protocol Biological specimens ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Helical Reconstruction Jump to Protocol Set ALLOWTOPICCHANGE ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Plunge Freezing Jump to Protocol Preservation of structure ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Electron Crystallography Jump to Principles 2dx subsub ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Electron Tomography Jump to Principles Imod / Etomo Protomo ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Single Particle Analysis Jump to Principles Spider EMAN ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Single Particle Analysis Jump to Protocol Three dimensional ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Negative Staining Jump to Protocol Why Negative stain ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Background of structural biology The objective of structural ...
Contents Reconstruction by Random Conical Tilt Principals For a very good description, see Frank (2006) Three Dimensional Electron Microscopy of Macromolecular ...
Contents Robot Instructions These instructions are not meant to replace proper training Preparation Start usage log Switch toggle to robot . ...
Courses and Seminars related to !CryoEM At the NYSBC CEM User meeting and Annual BBQ Event: CEM user meeting Location: NYSBC, Room A 11 Time: Friday, August 22, ...
(Archive)Courses and Seminars related to !CryoEM 2013 At the NYSBC Semester long Course : "Cryoelectron Microscopy of Macromolecular Assemblies" This comprehensive ...
Contents Ongoing issues Tecnai New apertures installed March 6 2015 Objective: 100 um, 50 um, 50 um, 40 um condenser: 150 um, 100 um, 70 um, 50 um BUT third ...
Procedure for using the CEM facility at the NYSBC The NYSBC The New York Structural Biology Center is a 501 (c)(3) corporation incorporated in the State of New York ...
Procedure for using the CEM facility at the NYSBC The NYSBC The New York Structural Biology Center is a 501 (c)(3) corporation incorporated in the State of New York ...
Single Particle Projects Random Conical Tilt (RCT) procedure methods used Oct 2012 Collect Data Prepare grids typically negative stain Use SerialEM to ...
Overview Interested in Cryoem? (How to begin a project) CemInterested Cryoem at NYSBC CemIntroduction General info on cryoem at NYSBC CemGeneralInfo ...
Contents Install download and install EMAN2 daily release in EMAN2/eman2.cshrc, Pythonpath needs to be corrected from default: setenv PYTHONPATH $ ...
Staff of the Cryoelectron Microscopy Facility Scientific Co Director: Bridget Carragher emg@nysbc.org tel: (212) 939 0660 ext 97241 fax: (212) 939 0863 webpage ...
How do I start? Note: The resources of the NYSBC are available for your use at no cost if you belong to a member institution. 1) Read thoroughly both the EM Introduction ...
How do I start? Note: The resources of the NYSBC are available for your use at no cost if you belong to a member institution. 1) Read thoroughly both the EM Introduction ...
Contents Montage tomograms using Protomo Collection In SerialEM, can collect montages at every tilt rather than single images To set up, choose File ...
Contents New Webpage Brief instructions for updating web pages manually ssh to cem100 Copy and paste the following commands to copy all pages: (~15 minutes ...
Contents Listing of twiki pages to be automatically copied to nysbc web pages Instructions List any pages here that you want to be mirrored on our public site ...
twikipage TemimpsJobs InstallwxPython PlungeFreezing CemProtocPlungefreezing PPPioloformGrids PPFiducialsTomography PPCarbonSandwich ...
CEMUserhandbook ver1.pdf: CEM User Handbook version 1.00 EM User Handbook Note: Policies may have been updated since this handbook has been completed so please contact ...
Welcome to the CEM home page SAFETY at NYSBC CEM Newsletter EM Related Seminars and Courses New EMG sites Leginon / Appion EMG site New scheduler ...
SAFETY Information for NYSBC EmergencyResponse All Hours info for NYSBC All Hours CEM Specific Info General Operating Handbook Chemical Safety ...
Contents Cold Stages You must be trained on proper handling of the cold stages before unattended use is permitted. Gatan This holder easily can obtain 1nm ...
Computer Processing File conversion, Fourier transformation, and plot CTF Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM DavidStokes 18 Mar 2005 ...
Contents March 2010 We will be given 1 month notice before final trench is dug. Anticipated date is not included in current construction schedules. Cryo EM As ...
Contents Vendor Contacts arranged by company name Name Position Company Phone email account number Markus ABRA Fluid/ work with ...
Overview of the NYSBC Core Facilities NMR Facility X ray Facility Protein production Facility NYCOMPS Electron crystallography Center The Transcontinental EM Initiative ...
CryoEM course evaluations Thank you for attending the course and thank you for submitting an evaluation. It will help us improve the course for next year. We ...
Current schedules of seminars and other public meetings related to Structural Biology for NYSBC. Please be advised that NYSBC web pages are the latest information ...
Equipment for Sample Preparation Sample Preparation for Cryo EM Equipment BOC Edwards Vacuum Evaporator HighPressFreezer PlungeFreezing Freeze ...
CryoUltraMicrotomy Microtome Conversion Cryosectioning notes from Chyongere Hirsch (Wadsworth Center Albany,NY)24 Mar 05 Visit the Cryo EM Web site at ...
Contents Recommended cshrc file setenv PATH /cryoem/fsu tomo/tomo 1.0.8 i686/bin/:/usr/bin:/usr/local/bin:/bin:/sbin:/usr/sbin:/cryoem/bin:/usr/kerberos/bin:/usr ...
Name: David Cowburn Login Name: cowburn Email: david.cowburn@einsteinmed.edu Location: Einstein Address: DavidCowburnAddress Einstein Web ...
My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest starting ...
Name: David Stokes Email: stokes@nyu.edu Company Name: NYU Company URL: http://stokeslab.med.nyu.edu Location: Skirball Institute 3 13 Country ...
Customise this topic; samples and ideas available at TWiki:TWiki.WebLeftBarCookbook. DavidStokes CemfacGroup CemAdmin CemProjects Reimbursement ...
Total PIs by facility Beam (a) Cryo EM NMR Total by facility Total Unique Total Users by facility Beam (a) Cryo ...
Total PIs by facility Beam (a) Cryo EM NMR Total by facility Total Unique Total Users by facility Beam (a) Cryo ...
Name: Deniz B Temel Occupation: PhD Student in Physics in The City University of New York, Graduate Center Email: dbtemel@gmail.com Company Name ...
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Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Dual Tilt axis tomography on the JEOL 3200FSC New How to use the dual tilt holders of the ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Contents Installation and Updating of EMAN2 Download and untar the latest release from http://blake.bcm.edu/emanwiki/EMAN2/Install/BinaryInstall go to installation ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest starting ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tecnai Tomography Contents This Software Is NO LONGER IN USE at NYSBC: Use SerialEM Microscope ...
Name: Fengxia Liang Email: liangf01@med.nyu.edu Company Name: NYU School of Medicine Company URL: http://saturn.med.nyu.edu Location: NYUSoMOffice ...
How to Plot 1D CTF profile of an image This can now be done more simply using EmIP Contents Setup Linux computer login startx depth 8 cd /dl380 ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Taking a film image Insert small screen adjust brightness until the exposure meter reads ...
Screening and Scanning EM Film Instructions for PDS 1010GM Microdensitometer Instructions for PDS 1010GM Data Processing Instructions for Zeiss Photoscan ...
Contents Instructions for finding the beam Move sample to make sure you are not on a grid bar If you are using the cryostage make sure the shutter is ...
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Name: FrankMacaluso Email: frank.macaluso@einstein.yu.edu Company Name: Albert Einstein College of Medicine Company URL: http://www.aecom.yu.edu/aif ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Freeze Substitution Jump to Principles Instructions for ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Principles Freeze Substitution Jump to Protocol Important considerations ...
Contents Processing of Tomograms using the FSU package fiducial free system new scripts automate much hand editing of files list of scripts setup ...
Contents Automated Control and Robotics on 1230 Options for Automated Control SerialEM already installed, but does not fully work because of emulator ...
GENERAL REFERENCE ITEMS FOR AFFILIATES AND STAFF AT NYSBC Frequently requested Intranet Pages Beamline facility page CEM facility page, ...
Glow Discharge in Edwards 306 Recommended that Glow Discharge be carried out by the dedicated units You will need to install the plasma ring for this procedure ...
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My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest starting ...
My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest starting ...
Contents Helical Processing : Initial steps display tube image and transform mrcdisp to display hft to calculate fft (must create id.box file with box ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Helical Reconstruction using EMIP This protocol uses software that originated ...
FEI Helios 650 The Helios is a state of the art SEM, equipped with a field emission gun and a focused ion beam, combining the very fine milling capabilities used in ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols High Pressure Freezing Jump to Principles Equipment Instructions ...
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Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Using the Bal Tec High Pressure Freezer Jump to Principles ...
Name: HyukSeo Email: hseo@rockefeller.edu Company Name: The Rockefeller University Company URL: Location: RockefellerUOffice Country: USA ...
Contents Ice Contamination Rate Test Theory All intensity measurements are proportional to number of electrons passing through sample. Measure them either directly ...
Return to Cryo EM Website at http://www.nysbc.org/facilities/CEM Cryo sample loading Contents Apertures Condenser aperture #2 Objective aperture #4 Check ...
Return to Cryo EM Website at http://www.nysbc.org/facilities/CEM Insert sample Open Vacuum Overview page Prepump Airlock Line up pin on holder ...
Contents 2DX Installation 2DX v2 Nov 14 2007 installed version 2.1 rpm does not work on any of our linux boxes gives floating point exception ...
Contents Prerequisites 1 python 1 gtk and glib. If the FSU software has been installed already then the required libraries should already be present 1 wxGTK ...
Value of all unique names at bottom of table Total unique Cryo EM PIs
Total PIs by facility Beam (a) Cryo EM NMR Total by facility Total Unique Total Users by facility Beam (a) Cryo ...
Total PIs by facility Beam (a) Cryo EM NMR Total by facility Total Unique Total Users by facility Beam (a) Cryo ...
Name: James Munro Email: jbm2002@med.cornell.edu Company Name: Weill Medical College of Cornell University Company URL: Location: WMCCUOffice ...
Customise this topic; samples and ideas available at TWiki:TWiki.WebLeftBarCookbook. My links: My home page My activities CemSamplePrepSchedule ...
Name: Jeffrey Bentley Email%: jbentley at wadsworth.org Company Name: The Wadsworth Institute Company URL: http://www.wadsworth.org Location: WadsworthOffice ...
JEOL 1230 The JEOL 1230 is mainly used as a screening microscope. It has a tungsten filament and a side entry Erlangshen Gatan camera mounted at the 35mm port. The ...
Contents JEOL 1230 Microscope Parameters wavelengths 80kV: 0.04176 A 120kV: 0.03349 A objective lenses f ?? Cs ?? mm Chromatic ...
Contents JEOL 1230 Instructions Log in User Name: su Password: 1230 Specimen Insertion Flip toggle switch to "pump" (G) Line up pin on holder ...
Contents User Instructions for 2100F Assumed that the microscope has already been aligned and software set up for imaging All users REQUIRE training with ...
JEOL 2100 The JEOL 2100F operates with an accelerating voltage of 200kV. It is equipped with a Schottky field emission gun and an array of three condenser lenses, ...
Contents JEOL 2100 Microscope Parameters wavelengths 200kV: 0.02508 A objective lenses f 2.8 mm Cs 2.0 mm Chromatic aberration 2.1 mm ...
JEOL 2100 Instructions Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM K2 Camera Regular Maintenance Anneal cycle should be run weekly ...
Contents User Instructions for 3200FSC These are for users who come to use the microscope Assumed that the microscope has already been aligned and software ...
JEOL 3200FSC The JEOL 3200FSC is a state of the art instrument that operates with an accelerating voltage of 300kV. It is equipped with a Schottky field emission gun ...
JEOL 3200 Microscope Parameters wavelengths 300kV: 0.01969 A objective lenses f 4.4 mm Cs 4.1 mm Chromatic aberration 3.4 mm Aperture ...
Contents JEOL 3200 training 3200 training notes.doc: DLS notes on 3200 training Cryogen fill Chuck dewar Fill in morning and last thing at night ...
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Name: Johanna Napetschnig Email: jnapetschn@mail.rockefeller.edu Company Name: The Rockefeller University Company URL: http://www.rockefeller.edu ...
Name: Jung Song Email: jhsong@chemail.engr.ccny.cuny.edu Company Name: Graduate Center of CUNY Company URL: http://www1.ccny.cuny.edu/prospective/engineering ...
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Name: Laura Barreyro Email SUSPENDED BOX FULL NO REPSONSE : barreyro@sci.ccny.cuny.edu Company Name: The City college of New York Company URL: http ...
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Name: Manolis Pahakis Email: ninemia@yahoo.com Company Name: Graduate Center CUNY (City College) Company URL: http://bme.ccny.cuny.edu/ Location ...
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META GROUP MEETINGS – NYSBC We would like to provide each participating group the opportunity to conduct a 'group meeting' at NYSBC in whatever format the group finds ...
Name: Michael Durney Email: mdurney AT rockefeller.edu Company Name: The Rockefeller University Company URL: http://www.rockefeller.edu Location ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Microtome Conversion Remove Cryosectioning unit Bake ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Contents Making Movies from Image Stills under Linux Required Software 1 ImageMagick for easy format conversion, cropping, etc. 1 mjpegtools for encoding ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Contents New User Setup Tecnai F20 Create new accounts on both computers and Greymatter Set passwords to never expire Windows 2000 or XP ...
My Project Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences ...
NIH Electronic Grant Submissions oppPA 07 070 cidVERSION 2A FORMS.xfd: unsolited R01 application file for !PureEdge oppPA 07 070 cidVERSION 2A FORMS instructions ...
Intra mural pages for NEW YORK STRUCTURAL BIOLOGY CENTER You will need to register as a NYSBC Intranet user at http://www.nysbc.net/twiki/register.html to get access ...
Various NYSBC Procedures New Affiliate Endorsement Procedures for setting up Teleconference and remote PC viewing Procedures for using Preapproved PO's ...
Contents RESOURCES (NYSBC GENERAL LABORATORIES, NIH style format, the individual sections can be copied and pasted ) FACILITIES: Specify the facilities ...
Total PIs by facility Beam (a) Cryo EM NMR Total by facility Total Unique Total Users by facility Beam (a) Cryo ...
Total PIs by facility Beam (a) Cryo EM NMR Total by facility Total Unique Total Users by facility Beam (a) Cryo ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Carbon Sandwich Jump to Principles You will need: ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Formvar Holey Film Jump to Principles This method has a ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Fiducial Markers for Tomography Jump to Principles Gold ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Negative Staining Jump to Principles You will need: ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Prepare Pioloform Grids Jump to Principles 1. Clean a ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Positive Staining Jump to Principles You will need: ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Solid Carbon Film Jump to Principles Using Mica (Best ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols TRIAFOL METHOD Jump to Principles Holey carbon grids ...
Name: ParthaDatta Email: ppd2k@yahoo.com Company Name: Wadsworth center Company URL: Location: WadsworthOffice Country: USA Comment ...
Contents Past EM Journal Clubs and seminar speakers 31 Oct 2011 4 pm Richard Henderson : MRC Laboratory of Molecular Biology, Cambridge, UK, "What is needed ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Contents Densitometer data Startx depth 8 Cd /home/nsfhome/kderr/pds … Change .HDR and .IMG files to lowercase Mv HDR filename.hdr Pds2mrc ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Using the PDS 1010GM Microdensitometer Download MSWord file ...
My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest starting ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Plastic Coated Grids Jump to Principles You will need ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Plunge freezing Jump to Principles You will need: ...
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Name: RajAgrawal Email: agrawal@wadsworth.org Company Name: Wadsworth Ctr Company URL: http://www. Location: WadsworthOffice Country: USA ...
Name: Raji Edayathumangalam Email: raji@brandeis.edu Company Name: Brandeis University Company URL: Location: Other Country: USA Comment ...
Name Option Hint Basic NMR: Needed for all NMR users; you will be contacted by NMR staff; you will need an NYSBC ID card Cryoprobe operations NMR ...
Name: Robert Grassucci Email: rg2502@columbia.edu Company Name:Columbia University Dept of Biochemistry and Molecular Biophysics Company URL: http: ...
My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest starting ...
My Links .NysbcIntro your introduction to NYSBC .ATasteOfTWiki view a short introductory presentation on TWiki for beginners .WelcomeGuest starting ...
Contents Group Page for the NYSBC Scientific Committee Specific areas (restricted to members) CEM section of scientific committee NMR section of ...
June 2008 Scientific Report, NYSBC Introduction In order to provide Principal Investigators and other interested parties a continuous overview of the scientific ...
Nov 2008 Scientific Report, NYSBC Figure contents described on last page. Introduction In order to provide Principal Investigators and ...
Current schedules of seminars and other public meetings related to Structural Biology for NYSBC. Please be advised that NYSBC web pages are the latest information ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM SerialEM Use Serial EM provided by David Mastronarde and colleagues from Univ. of Colorado, ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Contents Notes on Serial EM Use and Configuration on Tecnai F20 Steps to recording a Low Dose ...
Contents Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM To make a montage of your grid 1. Set magnification to 170X, maximum beam spread. ...
How to use Shutter box Control Application from TVIPS Background Tietz shutterbox has separate beam blank and camera shutter controls This allows beam to ...
Name: Shyam Bhaskaran Email: sbhaskaran@mail.rockefeller.edu Company Name: The Rockefeller University Company URL: http://www.rockefeller.edu/labheads ...
Contents Instructions for processing Single particle crystals 1 Setup directories: mkdir doc mkdir patch mkdir coran mkdir classavg 2 Convert from mrc ...
Contents List of Supported Software EM Image Processing Program Version Location Installed on cluster? Spider 15.10, 17.12 /cryoem/spider ...
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PROTOCOLS from Stokes Lab Members Cell Culture yeast growth protocol vink.doc: Yeast Culture (Martin Vink) 12/2007 Yeast cultures.doc: Yeast culture protocol ...
Contents Sample Insertion InsertRoomTemperatureSample InsertCryoSample Tecnai F20 Problems Instructions to Retrieve CCDBlankEnable Film Jam Access ...
Tecnai F20 The Tecnai F20 operates with an accelerating voltage of 200kV. It is equipped with a Schottky field emission gun. The tip on this instrument was replaced ...
FEI TF20 Microscope Parameters wavelengths 200kV: 0.02508 A objective lenses f Cs 2.1 mm Chromatic aberration Aperture Sizes Condensor ...
Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tecnai F20 (basic operations) TEM data computer controls Fast Scan CCD holds data and provides ...
Current Job Openings at NYSBC Entry level Technician Position in the Cryo Electron Microscopy Facility at the New York Structural Biology Center The Electron Microscopy ...
New York Structural Biology Center Sign Up to use the Cryoelectron Microscopy Facility Online Instrument Signup Online EM logbook New User registration: If ...
Name: TerryWagenknecht Email: terry@wadsworth.org Company Name: Wadsworth Ctr Company URL: http://www. Location: WadsworthOffice Country: ...
My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment preferences ...
Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tomography using TVIPS EM Menu 3.0 Contents This Software Is No Longer In Use at NYSBC : Use SerialEM ...
Name: Tieuvi Nguyen Email: tieuvi@gmail.com Company Name: The City College of New York Company URL: http://www. Location: CUNYCCNYOffice Country ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Tietz Tomography through EM Menu FEI Tomography software SerialEM tomography software Dual ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Edwards 306 Vacuum Evaporator Jump to Principles Carbon ...
Name: Wayne Hendrickson Email: wayne@convex.hhmi.columbia.edu Company Name: Columbia UP S Company URL: http://www.columbia.edu/cu/biology/faculty data ...
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Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Wohlwend Jump to Principles Start up Preparation ...
My Logbook My Links .NysbcIntro your introduction to NYSBC .NetLiterate short introduction to the style of this intranet Personal Preferences Uncomment ...
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Name: Yuan Tian Email: yut2002@med.cornell.edu Company Name: Sloan Kettering Institute Company URL: http://www. Location: MSKCCOffice Country ...
Screening and Scanning EM Film Procedure with screen shots available on web site http://www.nysbc.org/facilities/CEM/Zeiss PhotoScan Usage.html Download MSWord ...
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Name: Andres Baena Email: abaena@aecom.yu.edu Company Name: AECOM Company URL: http://www. Location: AECOMOffice Country: USA Comment ...
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